Pezato Rogério, Pérez-Novo Claudina A, Holtappels Gabriele, De Ruyck Natalie, Van Crombruggen Koen, De Vos Geert, Bachert Claus, Derycke Lara
Upper Airway Research Laboratory (URL), Department of Otorhinolaryngology, Ghent University Hospital, Ghent University, Belgium; Department of Otolaryngology-Head and Neck Surgery, Federal University of São Paulo, Brazil.
Upper Airway Research Laboratory (URL), Department of Otorhinolaryngology, Ghent University Hospital, Ghent University, Belgium.
Immunobiology. 2014 Sep;219(9):729-36. doi: 10.1016/j.imbio.2014.05.004. Epub 2014 Jun 4.
Chronic rhinosinusitis with nasal polyps (CRSwNP) is characterized as a Th2-driven disease. Activated dendritic cells (DCs) are the main T-cell activators; their role in the chronic inflammatory process of nasal polyposis is still unclear.
The regulation of DC subsets was analyzed in nasal polyp tissue from CRSwNP patients and compared to inferior turbinate tissue from healthy subjects. Tissue localization and expression of both plasmacytoid and myeloid DCs were assayed by means of immunohistochemistry and flow cytometry. Plasmacytoid DCs were also assayed by PCR, and tissue homogenates were assayed for various inflammatory markers.
The number of plasmacytoid (pDCs) and myeloid (mDCs) dendritic cells was significantly increased in nasal polyp tissue when compared to non-inflamed nasal mucosa. The number of pDCs, but not mDCs, was down-regulated in more severe cases (nasal polyps with asthma) and varied with the cytokine milieu. The amount of pDCs was significantly decreased in IL5+IFNγ - nasal polyp tissue compared to tissues with high IFNγ levels (IL5+IFNγ+). Furthermore, levels of indoleamine 2,3-dioxygenase were increased in nasal polyp compared to inferior turbinate tissue and correlated negatively with the number of pDCs.
There is an altered balance of pDC and mDC numbers in nasal polyp tissue. pDCs seem to be more susceptible to an inflammatory cytokine milieu and may play a crucial role in disease severity.
伴有鼻息肉的慢性鼻-鼻窦炎（CRSwNP）的特征是一种由Th2驱动的疾病。活化的树突状细胞（DCs）是主要的T细胞激活剂；它们在鼻息肉慢性炎症过程中的作用仍不清楚。
分析CRSwNP患者鼻息肉组织中DC亚群的调节情况，并与健康受试者的下鼻甲组织进行比较。通过免疫组织化学和流式细胞术检测浆细胞样和髓样DCs的组织定位和表达。通过PCR检测浆细胞样DCs，并用组织匀浆检测各种炎症标志物。
与未发炎的鼻黏膜相比，鼻息肉组织中浆细胞样（pDCs）和髓样（mDCs）树突状细胞的数量显著增加。在更严重的病例（伴有哮喘的鼻息肉）中，pDCs的数量下调，但mDCs的数量未下调，且其数量随细胞因子环境而变化。与IFNγ水平高的组织（IL5+IFNγ+）相比，IL5+IFNγ-鼻息肉组织中pDCs的数量显著减少。此外，与下鼻甲组织相比，鼻息肉中吲哚胺2,3-双加氧酶的水平升高，且与pDCs的数量呈负相关。
鼻息肉组织中pDC和mDC数量的平衡发生了改变。pDCs似乎更容易受到炎性细胞因子环境的影响，可能在疾病严重程度中起关键作用。
