Rana Suman, Jadhav Neena V, Barick K C, Pandey B N, Hassan P A
Chemistry Division, Bhabha Atomic Research Centre, Mumbai-400085, India.
Dalton Trans. 2014 Aug 28;43(32):12263-71. doi: 10.1039/c4dt00898g.
Superparamagnetic Fe3O4 nanoparticles are appealing materials for heat activated killing of cancer cells. Here, we report a novel method to enhance the heat activated killing of cancer cells under an AC magnetic field (AMF) by introducing a polyaniline impregnated shell onto the surface of Fe3O4 nanoparticles. These polyaniline shell cross-linked magnetic nanoparticles (PSMN) were prepared by in situ polymerization of aniline hydrochloride on the surface of carboxyl PEGylated Fe3O4 nanoparticles. XRD and TEM analyses revealed the formation of single phase inverse spinel Fe3O4 nanoparticles of a size of about 10 nm. The successful growth of the polyaniline shell on the surface of carboxyl PEGylated magnetic nanoparticles (CPMN) is evident from FTIR spectra, DLS, TGA, zeta-potential and magnetic measurements. Both CPMN and PSMN show good colloidal stability, superparamagnetic behavior at room temperature and excellent heating efficacy under AMF. It has been observed that the heating efficacy of PSMN under AMF was slightly reduced as compared to that of CPMN. The enhanced toxicity of PSMN to cancer cells under AMF suggests their strong potential for magnetic hyperthermia. Furthermore, PSMN shows high loading affinity for an anticancer drug (doxorubicin), its sustained release and substantial internalization in tumor cells.
超顺磁性Fe3O4纳米颗粒是用于热激活杀死癌细胞的有吸引力的材料。在此，我们报告了一种通过在Fe3O4纳米颗粒表面引入聚苯胺浸渍壳来增强在交流磁场（AMF）下热激活杀死癌细胞的新方法。这些聚苯胺壳交联磁性纳米颗粒（PSMN）是通过在羧基聚乙二醇化的Fe3O4纳米颗粒表面原位聚合盐酸苯胺制备的。XRD和TEM分析表明形成了尺寸约为10nm的单相反尖晶石Fe3O4纳米颗粒。从FTIR光谱、DLS、TGA、zeta电位和磁性测量可以明显看出聚苯胺壳在羧基聚乙二醇化磁性纳米颗粒（CPMN）表面成功生长。CPMN和PSMN都表现出良好的胶体稳定性、室温下的超顺磁性行为以及在AMF下优异的加热效果。据观察，与CPMN相比，PSMN在AMF下的加热效果略有降低。PSMN在AMF下对癌细胞的毒性增强表明它们在磁热疗方面具有强大的潜力。此外，PSMN对抗癌药物（阿霉素）表现出高负载亲和力、其缓释以及在肿瘤细胞中的大量内化。
