在戈亚尼亚市销售的米纳斯弗雷斯卡尔奶酪中进行大肠杆菌O157:H7的PCR和ELISA（VIDAS ECO O157(®)）鉴定。
PCR and ELISA (VIDAS ECO O157(®)) Escherichia coli O157:H7 identification in Minas Frescal cheese commercialized in Goiânia, GO.
作者信息
Carvalho Rosangela Nunes, de Oliveira Antonio Nonato, de Mesquita Albenones José, Minafra e Rezende Cíntia Silva, de Mesquita Adriano Queiroz, Romero Rolando Alfredo Mazzoni
机构信息
Embrapa Arroz e Feijão Santo Antônio de Goiás GoiásGO Brazil.
Centro de Pesquisa em Alimentos Escola de Veterinária e Zootecnia Universidade Federal de Goiás GoiâniaGO Brazil.
出版信息
Braz J Microbiol. 2014 May 19;45(1):7-10. doi: 10.1590/s1517-83822014000100002. eCollection 2014.
Escherichia coli O157:H7 has been incriminated in food poisoning outbreaks and sporadic cases of hemorrhagic colitis and hemolytic uremic syndrome in many countries. Considering the high susceptibility of Minas Frescal cheese to contamination by E. coli O157:H7, the aim of this study was to determine the occurrence of this pathogen through PCR (Polymerase Chain Reaction) and ELISA (VIDAS ECO O157(®), bioMérieux, Lyon, France) test. Thirty cheese samples manufactured by artisan farmhouse producers were collected from open-air markets in Goiânia and thirty from industries under Federal Inspection located in Goiás State which trade their products in supermarkets in Goiânia. E. coli O157:H7 was detected in 6.67% samples collected in open air markets using ELISA, and 23,33% with PCR. The pathogen was not detected in samples from industries under Federal Inspection.
在许多国家，肠出血性大肠杆菌O157:H7已被认定与食物中毒暴发以及散发性出血性结肠炎和溶血尿毒综合征病例有关。鉴于米纳斯弗雷斯科奶酪极易受到O157:H7大肠杆菌污染，本研究旨在通过聚合酶链反应（PCR）和酶联免疫吸附测定法（VIDAS ECO O157(®)，法国生物梅里埃公司，里昂）检测来确定该病原体的存在情况。从戈亚尼亚露天市场收集了30份由手工农家生产者制作的奶酪样品，以及从位于戈亚斯州且在戈亚尼亚超市销售产品的接受联邦检查的企业中收集了30份奶酪样品。使用酶联免疫吸附测定法在露天市场采集的样品中，6.67%检测出O157:H7大肠杆菌，而通过聚合酶链反应检测的比例为23.33%。在接受联邦检查的企业的样品中未检测到该病原体。
Zotero 插件