Ramos Carlos A N, Araújo Flábio R, Santos Rafaelle C, Melo Elaine S P, Sousa Letícia C, Vidal Carlos E S, Guerra Neurisvan R, Ramos Rafael A N
Embrapa Gado de Corte Campo GrandeMS Brazil.
Programa de Pós Graduação em Saúde Animal na Amazônia Universidade Federal do Pará CastanhalPA Brazil.
Braz J Microbiol. 2014 May 2;45(1):199-204. doi: 10.1590/S1517-83822014005000039. eCollection 2014.
The recombinant protein MSP5 has been established as an important antigen for serological diagnosis of Anaplasma marginale by enzyme-linked immunosorbent assay (ELISA). However, due to the high cost of specialized equipment, this technique is not accessible to all laboratories, especially in developing countries in areas where the disease is endemic. The present study describes the standardization of a latex agglutination test (LAT) to detect antibodies against A. marginale based on recombinant MSP5. Compared with indirect enzyme-linked immunosorbent assay (iELISA), the relative sensitivity and specificity of the LAT were 95.21% and 91.86% respectively, with an almost perfect agreement between tests (kappa index = 0.863). These results can be considered important for the serological diagnosis of A. marginale, as they indicate that the test represents a rapid and low cost alternative to ELISA.
重组蛋白MSP5已被确立为通过酶联免疫吸附测定（ELISA）对边缘无形体进行血清学诊断的重要抗原。然而，由于专用设备成本高昂，并非所有实验室都能采用这项技术，特别是在该疾病流行地区的发展中国家。本研究描述了基于重组MSP5的乳胶凝集试验（LAT）用于检测抗边缘无形体抗体的标准化方法。与间接酶联免疫吸附测定（iELISA）相比，LAT的相对敏感性和特异性分别为95.21%和91.86%，两种检测方法之间几乎完全一致（kappa指数 = 0.863）。这些结果对于边缘无形体的血清学诊断具有重要意义，因为它们表明该检测方法是一种快速且低成本的ELISA替代方法。
