Sansom Institute for Health Research, School of Pharmacy and Medical Sciences, University of South Australia, City East Campus, GPO Box 2471, Adelaide, SA 5001, Australia.
Sansom Institute for Health Research, School of Pharmacy and Medical Sciences, University of South Australia, City East Campus, GPO Box 2471, Adelaide, SA 5001, Australia ; Discipline of Physiology, School of Medical Sciences, University of Adelaide, Adelaide, SA 5005, Australia.
Int J Endocrinol. 2014;2014:916918. doi: 10.1155/2014/916918. Epub 2014 May 14.
Low birth weight is associated with reduced bone mass and density in adult life. However, effects of maternal hypoxia (MH) on offspring bone development are not known. Objective. The current study investigated the effects of fetal growth restriction induced by MH during the last half of gestation on bone structure and volume in the offspring of the fetus near term and the pup in adolescence. Methods. During 35-62-day gestation (term, 69d), guinea pigs were housed in room air (21% O2; control) or 12% O2 (MH). Offspring femur and tibia were collected at 62d gestation and 120d after birth. Results. MH decreased fetal birth weight but did not affect osteogenic potential pools in the fetal bone marrow. Histological analysis showed no effects of MH on tibial growth plate thickness in either fetal or postnatal offspring, although there was increased VEGF mRNA expression in the growth plate of postnatal offspring. MH did not change primary spongiosa height but lowered collagen-1 mRNA expression in postnatal offspring. There was increased mRNA expression of adipogenesis-related gene (FABP4) in bone from the MH postnatal offspring. Conclusion. MH during late gestation did not change the pool of osteogenic cells before birth or growth plate heights before and after birth. However, MH reduced expression of bone formation marker (collagen-1) and increased expression of fat formation marker (FABP4) in postnatal offspring bone.
低出生体重与成年后骨量和骨密度降低有关。然而，母体缺氧 (MH) 对子代骨骼发育的影响尚不清楚。目的。本研究探讨了妊娠晚期 MH 诱导的胎儿生长受限对近足月胎儿和青春期幼仔骨结构和体积的影响。方法。在 35-62 天的妊娠期（足月，69d）期间，豚鼠被饲养在空气中（21% O2；对照组）或 12% O2（MH）中。在妊娠 62 天和出生后 120 天收集后代股骨和胫骨。结果。MH 降低了胎儿的出生体重，但不影响胎儿骨髓中的成骨潜能池。组织学分析表明，MH 对胎儿和产后后代胫骨生长板厚度均无影响，尽管产后后代生长板中 VEGF mRNA 表达增加。MH 并未改变初级海绵骨的高度，但降低了产后后代的胶原蛋白-1 mRNA 表达。来自 MH 产后后代的骨中脂肪生成相关基因（FABP4）的 mRNA 表达增加。结论。妊娠晚期的 MH 并没有改变出生前的成骨细胞池或出生前后生长板的高度。然而，MH 降低了产后后代骨中骨形成标志物（胶原蛋白-1）的表达，并增加了脂肪形成标志物（FABP4）的表达。
