United States Department of Agriculture, Agricultural Research Service, Arthropod-Borne Animal Diseases Research Unit , Manhattan, KS , USA.
PeerJ. 2014 Jun 5;2:e426. doi: 10.7717/peerj.426. eCollection 2014.
Culicoides biting midges (Diptera: Ceratopogonidae) are hematophagous insects with over 1400 species distributed throughout the world. Many of these species are of particular agricultural importance as primary vectors of bluetongue and Schmallenberg viruses, yet little is known about Culicoides genomics and proteomics. Detailed studies of members from other blood-feeding Dipteran families, including those of mosquito (Culicidae) and black fly (Simuliidae), have shown that protein components within the insect's saliva facilitate the blood feeding process. To determine the protein components in Culicoides sonorensis midges, secreted saliva was collected for peptide sequencing by tandem mass spectrometry. Forty-five secreted proteins were identified, including members of the D7 odorant binding protein family, Kunitz-like serine protease inhibitors, maltase, trypsin, and six novel proteins unique to C. sonorensis. Identifying the complex myriad of proteins in saliva from blood-feeding Dipteran species is critical for understanding their role in blood feeding, arbovirus transmission, and possibly the resulting disease pathogenesis.
致倦库蚊（双翅目：蠓科）是吸血昆虫，分布于世界各地，有超过 1400 种。其中许多物种对农业具有特别重要的意义，因为它们是蓝舌病病毒和 Schmallenberg 病毒的主要传播媒介，但对致倦库蚊的基因组学和蛋白质组学知之甚少。对其他吸血双翅目家族成员的详细研究，包括蚊子（蚊科）和黑蝇（蚋科），表明昆虫唾液中的蛋白质成分有助于吸血过程。为了确定索诺兰蠓的蛋白质成分，收集了分泌的唾液进行串联质谱肽测序。鉴定出 45 种分泌蛋白，包括 D7 气味结合蛋白家族成员、Kunitz 样丝氨酸蛋白酶抑制剂、麦芽糖酶、胰蛋白酶和 6 种索诺兰蠓特有的新型蛋白。鉴定吸血双翅目物种唾液中的复杂蛋白质群对于了解它们在吸血、虫媒病毒传播以及可能由此产生的疾病发病机制中的作用至关重要。
