Sasidharan Nishanth Kumar, Sreekala Sreerag Ravikumar, Jacob Jubi, Nambisan Bala
Agroprocessing and Natural Products Division, National Institute for Interdisciplinary Science and Technology (NIIST), Council of Scientific and Industrial Research (CSIR), Thiruvananthapuram, Kerala 695 019, India.
Division of Crop Protection and Division of Crop Utilization, Central Tuber Crops Research Institute, Sreekariyam, Thiruvananthapuram 695017, India.
Biomed Res Int. 2014;2014:561456. doi: 10.1155/2014/561456. Epub 2014 May 18.
Diarrhea is one of the leading causes of morbidity and mortality in humans in developed and developing countries. Furthermore, increased resistance to antibiotics has resulted in serious challenges in the treatment of this infectious disease worldwide. Therefore, there exists a need to develop alternative natural or combination drug therapies. The aim of the present study was to investigate the synergistic effect of curcumin-1 in combination with three antibiotics against five diarrhea causing bacteria. The antibacterial activity of curcumin-1 and antibiotics was assessed by the broth microdilution method, checkerboard dilution test, and time-kill assay. Antimicrobial activity of curcumin-1 was observed against all tested strains. The MICs of curcumin-1 against test bacteria ranged from 125 to 1000 μ g/mL. In the checkerboard test, curcumin-1 markedly reduced the MICs of the antibiotics cefaclor, cefodizime, and cefotaxime. Significant synergistic effect was recorded by curcumin-1 in combination with cefotaxime. The toxicity of curcumin-1 with and without antibiotics was tested against foreskin (FS) normal fibroblast and no significant cytotoxicity was observed. From our result it is evident that curcumin-1 enhances the antibiotic potentials against diarrhea causing bacteria in in vitro condition. This study suggested that curcumin-1 in combination with antibiotics could lead to the development of new combination of antibiotics against diarrhea causing bacteria.
腹泻是发达国家和发展中国家人类发病和死亡的主要原因之一。此外，抗生素耐药性的增加给全球这种传染病的治疗带来了严峻挑战。因此，需要开发替代的天然药物或联合药物疗法。本研究的目的是研究姜黄素 -1 与三种抗生素联合使用对五种引起腹泻的细菌的协同作用。通过肉汤微量稀释法、棋盘稀释试验和时间杀菌试验评估姜黄素 -1 和抗生素的抗菌活性。观察到姜黄素 -1 对所有测试菌株均有抗菌活性。姜黄素 -1 对测试细菌的最低抑菌浓度范围为 125 至 1000μg/mL。在棋盘试验中，姜黄素 -1 显著降低了头孢克洛、头孢地嗪和头孢噻肟的最低抑菌浓度。姜黄素 -1 与头孢噻肟联合使用具有显著的协同作用。测试了含抗生素和不含抗生素的姜黄素 -1 对包皮（FS）正常成纤维细胞的毒性，未观察到明显的细胞毒性。从我们的结果可以明显看出，姜黄素 -1 在体外条件下增强了抗生素对引起腹泻细菌的作用。这项研究表明，姜黄素 -1 与抗生素联合使用可能会导致开发出针对引起腹泻细菌的新型抗生素组合。
