编码小鼠酪氨酸酶的变异cDNA的分离与鉴定。

Isolation and characterization of variant cDNAs encoding mouse tyrosinase.

作者信息

Terao M, Tabe L, Garattini E, Sartori D, Studer M, Mintz B

机构信息

Molecular Biology Unit, Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.

出版信息

Biochem Biophys Res Commun. 1989 Mar 15;159(2):848-53. doi: 10.1016/0006-291x(89)90072-7.

DOI:10.1016/0006-291x(89)90072-7

PMID:2494997

Abstract

Two different cDNA clones encoding mouse tyrosinase (monophenol oxygenase, E.C. 1.14.18.1) were isolated from B16 melanoma cells, and their primary structure was determined. One of the cDNAs consists of 3309 nucleotides with an open reading frame coding for a peptide of 533 amino acids. The other cDNA is approximately 1600 nucleotides long, with a shorter 3'-untranslated region and a deduced in-frame deletion of 77 amino acid residues with respect to the former clone. Neither of these clones is structurally identical to other described mouse tyrosinase cDNAs (1-3). RNA blotting analysis demonstrates that multiple tyrosinase mRNA species are not only present in B16 melanoma, but also in normal skin melanocytes.

摘要

从B16黑色素瘤细胞中分离出两个编码小鼠酪氨酸酶（单酚氧化酶，E.C. 1.14.18.1）的不同cDNA克隆，并确定了它们的一级结构。其中一个cDNA由3309个核苷酸组成，有一个开放阅读框，编码一个533个氨基酸的肽。另一个cDNA约1600个核苷酸长，3'-非翻译区较短，相对于前一个克隆，推断有一个77个氨基酸残基的框内缺失。这些克隆在结构上均与其他已描述的小鼠酪氨酸酶cDNA（1-3）不同。RNA印迹分析表明，多种酪氨酸酶mRNA不仅存在于B16黑色素瘤中，也存在于正常皮肤黑素细胞中。