脐带血 CD4(+)CD25(+) 调节性 T 细胞由于 TGF-β1 的产生和表达不足，无法抑制脐带血 NK 细胞的功能。
Cord blood CD4(+)CD25(+) regulatory T cells fail to inhibit cord blood NK cell functions due to insufficient production and expression of TGF-beta1.
机构信息
Clinical Research Institute, Mie Chuo Medical Center, National Hospital Organization, Tsu, Mie, Japan; Department of Neonatal Science, Institute of Molecular and Experimental Medicine, Mie University Graduate School of Medicine, Tsu, Mie, Japan; Shanghai Children's Medical Center, Shanghai, China.
Clinical Research Institute, Mie Chuo Medical Center, National Hospital Organization, Tsu, Mie, Japan; Department of Neonatal Science, Institute of Molecular and Experimental Medicine, Mie University Graduate School of Medicine, Tsu, Mie, Japan.
出版信息
Cell Immunol. 2014 Jul;290(1):89-95. doi: 10.1016/j.cellimm.2014.05.007. Epub 2014 Jun 2.
Although CD4(+)CD25(+) Treg (Treg) cells are known to modulate NK cell functions, the modulation mechanism of these cells in cord blood has not been fully clarified. The purpose of this study was to clarify the mechanism whereby cord blood Treg cells modulate cord NK cells. By performing various cultures of purified NK cells with or without autologous Treg cells, diminished inhibitory effects of cord Treg cells towards cord NK cell functions, including activation, cytokine production, and cytotoxicity, were observed. We also observed lower secretion of sTGF-beta1 and lower expression of mTGF-beta1 by cord Treg cells than by adult Treg cells. These data revealed the capability of adult Treg cells to suppress rhIL-2-stimulated NK cell function by TGF-beta1, both membrane-bound and soluble types. The reduced inhibitory capabilities of cord Treg cells compared with adult Treg cells is thought to be due to insufficient expression of TGF-beta1.
虽然已知 CD4(+)CD25(+)Treg（调节性 T 细胞）细胞可调节 NK 细胞的功能，但这些细胞在脐血中的调节机制尚未完全阐明。本研究旨在阐明脐血 Treg 细胞调节脐血 NK 细胞的机制。通过对纯化的 NK 细胞进行各种培养，包括与自体 Treg 细胞共培养和不共培养，观察到脐血 Treg 细胞对脐血 NK 细胞功能的抑制作用减弱，包括激活、细胞因子产生和细胞毒性。我们还观察到脐血 Treg 细胞分泌的 sTGF-beta1 和 mTGF-beta1 均低于成人 Treg 细胞。这些数据揭示了成人 Treg 细胞通过 TGF-beta1（膜结合型和可溶性型）抑制 rhIL-2 刺激的 NK 细胞功能的能力。与成人 Treg 细胞相比，脐血 Treg 细胞的抑制能力降低，可能是由于 TGF-beta1 的表达不足所致。
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