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原发性肝癌患者外周血中 CD4+CD25+CD127 调节性 T 细胞及细胞因子的表达

Expression of CD4+CD25+CD127 regulatory T cells and cytokines in peripheral blood of patients with primary liver carcinoma.

机构信息

Clinical laboratory, the First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510080, China.

Department of Endocrinology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Health Science Center of Shenzhen University, Shenzhen 518035, People's Republic of China.

出版信息

Int J Med Sci. 2020 Feb 24;17(6):712-719. doi: 10.7150/ijms.44088. eCollection 2020.

DOI:10.7150/ijms.44088
PMID:32218692
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7085268/
Abstract

To assess the clinical utility of the ratio of CD4+CD25+CD127 regulatory T cells (Tregs) in subjects at high risk of HCC, investigate the relationship between the percentage of Tregs and the expression of transforming growth factor (TGF)-β1 and interleukin (IL)-10 in patients with hepatocellular carcinoma before and after treatment. Peripheral venous blood was collected from patients with liver cancer before and after treatment. The proportion of CD4+CD25+CD127 Tregs was detected by flow cytometry. The levels of TGF-β1 and IL-10 in serum were detected by enzyme-linked immunosorbent assay, and were compared with healthy subjects as a control group. The proportion of CD4+CD25+CD127 to CD4+T lymphocytes in patients with hepatocellular carcinoma was significantly higher than that in healthy controls (). The proportion of CD4+CD25+CD127Tregs, whose AUC of ROC curve was 0.917, could effectively separate the HCC patients from the healthy subjects with a diagnostic sensitivity of 90%, specificity of 80%. The proportion of CD4+CD25+CD127 to CD4+T lymphocytes and the levels of TGF-β1 and IL-10 in patients with hepatocellular carcinoma after the operation and chemotherapy were significantly lower than those before treatment (P<0.05).The proportion of CD4+CD25+CD127Tregs was positively correlated with the concentrations of TGF-β1 and IL-10 before and after treatment of primary liver cancer (P<0.05). CD4+CD25+CD127Tregs may be a significant predictor of HCC biopsy outcome and play an inhibitory role on effector T cells by regulating cytokines.

摘要

为了评估 CD4+CD25+CD127 调节性 T 细胞（Tregs）在 HCC 高危人群中的临床应用价值，我们研究了肝癌患者治疗前后 Tregs 比例与转化生长因子（TGF）-β1 和白细胞介素（IL）-10 表达之间的关系。采集肝癌患者治疗前后的外周静脉血，采用流式细胞术检测 CD4+CD25+CD127 Tregs 的比例，酶联免疫吸附试验（ELISA）检测血清中 TGF-β1 和 IL-10 的水平，并与健康对照组进行比较。结果显示，肝癌患者 CD4+CD25+CD127 对 CD4+T 淋巴细胞的比例明显高于健康对照组（）。CD4+CD25+CD127Tregs 比例的 AUC 为 0.917，可有效区分 HCC 患者与健康对照者，诊断敏感度为 90%，特异度为 80%。肝癌患者手术后和化疗后的 CD4+CD25+CD127Tregs 比例以及 TGF-β1 和 IL-10 水平明显低于治疗前（P<0.05）。CD4+CD25+CD127Tregs 比例与原发性肝癌治疗前后 TGF-β1 和 IL-10 的浓度呈正相关（P<0.05）。CD4+CD25+CD127Tregs 可能是 HCC 活检结果的重要预测指标，通过调节细胞因子对效应 T 细胞发挥抑制作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/551a/7085268/57e010bc4311/ijmsv17p0712g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/551a/7085268/ad088c6b2acd/ijmsv17p0712g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/551a/7085268/4393f4f63ed9/ijmsv17p0712g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/551a/7085268/57e010bc4311/ijmsv17p0712g007.jpg

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