Flavell D J, Wright D H
University Department of Pathology, Southampton General Hospital, UK.
Br J Cancer. 1989 Feb;59(2):165-73. doi: 10.1038/bjc.1989.35.
The properties of rosettes formed between the Hodgkin's cell lines, L428 and L591, and allogeneic peripheral blood mononuclear cell populations have been investigated. Immunocytochemical analysis showed that the majority of adherent cells were T-cells of both the CD4 and CD8 subsets. Only relatively few B-cells and monocytes were seen to adhere. However, when peripheral blood mononuclear cell populations were fractionated, it was found that monocytes were as good as T-cells at forming rosettes with both L428 and L591, though B-cells were shown to be poor at forming such associations. Treatment of both L428 and L591 with neuraminidase resulted in a significant reduction (P less than 0.01) in the mean number of adherent lymphocytes and in the numbers of Hodgkin's tumour cells which formed rosettes. Smaller, less significant effects were observed for Cytochalasin B and trypsin. EDTA (10(-2) M) at pH 7.2 had no significant effect on rosetting for L428 or L591. Adherence of allogeneic lymphocytes to L428 or L591 was pH dependent but did not appear to correlate with cell surface charge. Treatment of L428 cells with Fab fragments prepared from the IgG fraction of a hyperimmune rabbit anti-L428 antiserum, significantly (P less than 0.05) inhibited the adherence of allogeneic lymphocytes, but only when used at high concentration. The binding requirements of the Hodgkin's cell lines with allogeneic peripheral blood lymphocytes, as described in this study, appear to be quite different from those described for freshly isolated Hodgkin's tumour cells with autologous intratumoral lymphocytes. This suggests that the two phenomena may be unrelated. There would appear to be an absolute requirement for cell surface sialic acid for allogeneic lymphocyte attachment to the HD cell lines. This might suggest that the receptor-ligand system involved contains sialic acid as an integral part of the cell surface receptor structure involved in recognition of the appropriate ligand.
对霍奇金细胞系L428和L591与异基因外周血单个核细胞群体形成的玫瑰花结的特性进行了研究。免疫细胞化学分析表明，大多数贴壁细胞是CD4和CD8亚群的T细胞。仅见相对较少的B细胞和单核细胞贴壁。然而，当对外周血单个核细胞群体进行分级分离时，发现单核细胞与T细胞一样，与L428和L591都能很好地形成玫瑰花结，尽管B细胞显示出形成这种结合的能力较差。用神经氨酸酶处理L428和L591均导致贴壁淋巴细胞的平均数量以及形成玫瑰花结的霍奇金肿瘤细胞数量显著减少（P小于0.01）。观察到细胞松弛素B和胰蛋白酶的作用较小且不显著。pH 7.2的10（-2）M乙二胺四乙酸（EDTA）对L428或L591的玫瑰花结形成无显著影响。异基因淋巴细胞与L428或L591的贴壁呈pH依赖性，但似乎与细胞表面电荷无关。用从超免疫兔抗L428抗血清的IgG组分制备的Fab片段处理L428细胞，显著（P小于0.05）抑制异基因淋巴细胞的贴壁，但仅在高浓度使用时才有效。本研究中描述的霍奇金细胞系与异基因外周血淋巴细胞的结合要求，似乎与新鲜分离的霍奇金肿瘤细胞与自体肿瘤内淋巴细胞的结合要求有很大不同。这表明这两种现象可能无关。异基因淋巴细胞附着于HD细胞系似乎绝对需要细胞表面唾液酸。这可能表明所涉及的受体 - 配体系统包含唾液酸作为参与识别适当配体的细胞表面受体结构的组成部分。
