Diehl V, Kirchner H H, Burrichter H, Stein H, Fonatsch C, Gerdes J, Schaadt M, Heit W, Uchanska-Ziegler B, Ziegler A, Heintz F, Sueno K
Cancer Treat Rep. 1982 Apr;66(4):615-32.
In the last 3 years we were able to establish five long-term in vitro cell cultures from biopsy specimens taken preterminally from four patients with histologically proven Hodgkin's disease (nodular sclerosing type, clinical stage IVB). Four of the lines are continuously proliferating in vitro; one culture stopped growth for unknown reasons after 7 months. When culture conditions were modulated, the first culture, L 428, gave rise to two sublines: L 428 KS, after adaptation to calf serum, and L 428 KSA, permanently growing as an adherent monolayer line after treatment with a phorbol ester (12-O-tetradecanoylphorbol-13-acetate) for 3 weeks. Cell-marker analysis by conventional means (SIg, cIg, rosette formation, Epstein-barr virus reactivity, cytochemistry, phagocytosis, and lysozyme production) and with monoclonal antibodies directed against various human lymphoid, myeloid, and monocytoid antigens showed that the tested cell lines are clearly different from all hitherto described hematopoietic lines; they most likely represent a cell type resembling an early myeloid-monocytoid progenitor cell. Conditioned medium of the L 428 cells and its two sublines showed colony-stimulating factor activity and suppression of spontaneous cell-mediated cytolysis of L 428 KS and K 562 cells.
在过去3年里，我们从4例经组织学证实为霍奇金病（结节硬化型，临床IVB期）患者临终前获取的活检标本中成功建立了5种长期体外细胞培养物。其中4种细胞系在体外持续增殖；一种培养物在7个月后因不明原因停止生长。当调整培养条件时，第一种培养物L 428产生了两个亚系：适应小牛血清后的L 428 KS，以及在用佛波酯（12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯）处理3周后作为贴壁单层细胞系永久生长的L 428 KSA。通过常规方法（表面免疫球蛋白、胞质免疫球蛋白、玫瑰花结形成、爱泼斯坦 - 巴尔病毒反应性、细胞化学、吞噬作用和溶菌酶产生）以及针对各种人类淋巴细胞、髓细胞和单核细胞抗原的单克隆抗体进行的细胞标志物分析表明，所测试的细胞系与迄今描述的所有造血细胞系明显不同；它们很可能代表一种类似于早期髓样 - 单核样祖细胞的细胞类型。L 428细胞及其两个亚系的条件培养基显示出集落刺激因子活性，并抑制L 428 KS和K 562细胞的自发细胞介导的细胞溶解。
