• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

一种适用于小脑颗粒细胞中诱导性基因操作的Nse-CreERT2转基因系的构建与鉴定

Generation and characterization of an Nse-CreERT2 transgenic line suitable for inducible gene manipulation in cerebellar granule cells.

作者信息

Pohlkamp Theresa, Steller Laura, May Petra, Günther Thomas, Schüle Roland, Frotscher Michael, Herz Joachim, Bock Hans H

机构信息

Center for Neuroscience, Department of Neuroanatomy, Albert-Ludwigs-University, Freiburg, Germany; Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America.

Center for Neuroscience, Department of Neuroanatomy, Albert-Ludwigs-University, Freiburg, Germany.

出版信息

PLoS One. 2014 Jun 20;9(6):e100384. doi: 10.1371/journal.pone.0100384. eCollection 2014.

DOI:10.1371/journal.pone.0100384
PMID:24950299
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4065071/
Abstract

We created an Nse-CreERT2 mouse line expressing the tamoxifen-inducible CreERT2 recombinase under the control of the neuron-specific enolase (Nse) promoter. By using Cre reporter lines we could show that this Nse-CreERT2 line has recombination activity in the granule cells of all cerebellar lobules as well as in postmitotic granule cell precursors in the external granular layer of the developing cerebellum. A few hippocampal dentate gyrus granule cells showed Cre-mediated recombination as well. Cre activity could be induced in both the developing and adult mouse brain. The established mouse line constitutes a valuable tool to study the function of genes expressed by cerebellar granule cells in the developing and adult brain. In combination with reporter lines it is a useful model to analyze the development and maintenance of the cerebellar architecture including granule cell distribution, migration, and the extension of granule cell fibers in vivo.

摘要

我们构建了一种Nse-CreERT2小鼠品系，该品系在神经元特异性烯醇化酶（Nse）启动子的控制下表达他莫昔芬诱导型CreERT2重组酶。通过使用Cre报告基因品系，我们可以证明该Nse-CreERT2品系在所有小脑小叶的颗粒细胞以及发育中小脑外颗粒层的有丝分裂后颗粒细胞前体中具有重组活性。少数海马齿状回颗粒细胞也显示出Cre介导的重组。Cre活性可在发育中和成年小鼠大脑中诱导产生。所建立的小鼠品系是研究发育中和成年大脑中小脑颗粒细胞表达的基因功能的宝贵工具。与报告基因品系结合使用时，它是一种有用的模型，可用于分析小脑结构的发育和维持，包括颗粒细胞分布、迁移以及体内颗粒细胞纤维的延伸。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3cc/4065071/444becf2191e/pone.0100384.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3cc/4065071/f03df47e2f00/pone.0100384.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3cc/4065071/a706ec5f509a/pone.0100384.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3cc/4065071/076da6bb97ec/pone.0100384.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3cc/4065071/42367f0c773b/pone.0100384.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3cc/4065071/e36fdc212c86/pone.0100384.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3cc/4065071/0d7ab79a5fc5/pone.0100384.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3cc/4065071/807347937519/pone.0100384.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3cc/4065071/444becf2191e/pone.0100384.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3cc/4065071/f03df47e2f00/pone.0100384.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3cc/4065071/a706ec5f509a/pone.0100384.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3cc/4065071/076da6bb97ec/pone.0100384.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3cc/4065071/42367f0c773b/pone.0100384.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3cc/4065071/e36fdc212c86/pone.0100384.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3cc/4065071/0d7ab79a5fc5/pone.0100384.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3cc/4065071/807347937519/pone.0100384.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a3cc/4065071/444becf2191e/pone.0100384.g008.jpg

相似文献

1
Generation and characterization of an Nse-CreERT2 transgenic line suitable for inducible gene manipulation in cerebellar granule cells.一种适用于小脑颗粒细胞中诱导性基因操作的Nse-CreERT2转基因系的构建与鉴定
PLoS One. 2014 Jun 20;9(6):e100384. doi: 10.1371/journal.pone.0100384. eCollection 2014.
2
Inducible gene manipulations in brain serotonergic neurons of transgenic rats.诱导型基因操作在转基因大鼠脑 5-羟色胺能神经元中的应用。
PLoS One. 2011;6(11):e28283. doi: 10.1371/journal.pone.0028283. Epub 2011 Nov 29.
3
Cre-mediated transgene activation in the developing and adult mouse brain.Cre介导的转基因在发育中和成年小鼠大脑中的激活。
Genesis. 2001 Nov;31(3):118-25. doi: 10.1002/gene.10014.
4
Astroglial cells in the external granular layer are precursors of cerebellar granule neurons in neonates.外颗粒层中的星形胶质细胞是新生儿小脑颗粒神经元的前体细胞。
Mol Cell Neurosci. 2010 Aug;44(4):362-73. doi: 10.1016/j.mcn.2010.05.001. Epub 2010 May 12.
5
Cerebellar granule cell-specific and inducible expression of Cre recombinase in the mouse.小鼠中Cre重组酶在小脑颗粒细胞中的特异性及可诱导表达。
J Neurosci. 1999 Dec 1;19(23):10318-23. doi: 10.1523/JNEUROSCI.19-23-10318.1999.
6
Tamoxifen-inducible NaV1.8-CreERT2 recombinase activity in nociceptive neurons of dorsal root ganglia.他莫昔芬诱导的背根神经节伤害性神经元中NaV1.8-CreERT2重组酶活性
Genesis. 2006 Aug;44(8):364-71. doi: 10.1002/dvg.20224.
7
Generation of NSE-MerCreMer transgenic mice with tamoxifen inducible Cre activity in neurons.生成神经元中可诱导 Cre 活性的 NSE-MerCreMer 转基因小鼠。
PLoS One. 2012;7(5):e35799. doi: 10.1371/journal.pone.0035799. Epub 2012 May 7.
8
Inducible Cre recombinase activity in mouse cerebellar granule cell precursors and inner ear hair cells.小鼠小脑颗粒细胞前体和内耳毛细胞中的诱导型Cre重组酶活性。
Dev Dyn. 2006 Nov;235(11):2991-8. doi: 10.1002/dvdy.20948.
9
Split-CreERT2: temporal control of DNA recombination mediated by split-Cre protein fragment complementation.Split-CreERT2：通过 Cre 蛋白片段互补实现的 DNA 重组的时间控制。
PLoS One. 2009 Dec 16;4(12):e8354. doi: 10.1371/journal.pone.0008354.
10
Neuron-specific enolase-cre mouse line with cre activity in specific neuronal populations.在特定神经元群体中具有cre活性的神经元特异性烯醇化酶-cre小鼠品系。
Genesis. 2006 Mar;44(3):130-5. doi: 10.1002/gene.20197.

引用本文的文献

1
A molecular brake that modulates spliceosome pausing at detained introns contributes to neurodegeneration.一种调节内含子滞留时剪接体暂停的分子刹车，有助于神经退行性变。
Protein Cell. 2023 May 8;14(5):318-336. doi: 10.1093/procel/pwac008.
2
Magnetic resonance imaging focused on the ferritin heavy chain 1 reporter gene detects neuronal differentiation in stem cells.聚焦于铁蛋白重链1报告基因的磁共振成像可检测干细胞中的神经元分化。
Neural Regen Res. 2023 Jul;18(7):1563-1569. doi: 10.4103/1673-5374.358608.
3
Protocol for sleep analysis in the brain of genetically modified adult mice.

本文引用的文献

1
Tamoxifen-Induced Cre-loxP Recombination Is Prolonged in Pancreatic Islets of Adult Mice.他莫昔芬诱导的 Cre-loxP 重组在成年小鼠胰岛中持续时间较长。
PLoS One. 2012;7(3):e33529. doi: 10.1371/journal.pone.0033529. Epub 2012 Mar 28.
2
Remodeling of monoplanar Purkinje cell dendrites during cerebellar circuit formation.在小脑回路形成过程中单平面浦肯野细胞树突的重塑。
PLoS One. 2011;6(5):e20108. doi: 10.1371/journal.pone.0020108. Epub 2011 May 31.
3
A robust and high-throughput Cre reporting and characterization system for the whole mouse brain.
用于分析成年转基因小鼠大脑中睡眠的方案。
STAR Protoc. 2021 Dec 8;2(4):100982. doi: 10.1016/j.xpro.2021.100982. eCollection 2021 Dec 17.
4
Cell Type-specific Knockout with -mediated Cre Recombination in the Developing Cerebellum.在发育中的小脑中通过介导的Cre重组进行细胞类型特异性基因敲除。
Exp Neurobiol. 2021 Jun 30;30(3):203-212. doi: 10.5607/en21017.
5
Fat cadherins in mouse models of degenerative ataxias.脂肪钙黏蛋白在退行性共济失调的小鼠模型中的作用。
Sci Rep. 2019 Nov 6;9(1):16155. doi: 10.1038/s41598-019-52684-7.
6
Viral Vector-Based Evaluation of Regulatory Regions in the Neuron-Specific Enolase (NSE) Promoter in Mouse Cerebellum In Vivo.病毒载体在体内对小脑神经元特异性烯醇化酶（NSE）启动子调控区的评估。
Cerebellum. 2017 Dec;16(5-6):913-922. doi: 10.1007/s12311-017-0866-5.
7
Inflammation-induced reversible switch of the neuron-specific enolase promoter from Purkinje neurons to Bergmann glia.炎症诱导神经元特异性烯醇化酶启动子从浦肯野神经元向伯格曼胶质细胞的可逆性转换。
Sci Rep. 2016 Jun 13;6:27758. doi: 10.1038/srep27758.
8
Heterogeneous transgene expression in the retinas of the TH-RFP, TH-Cre, TH-BAC-Cre and DAT-Cre mouse lines.TH-RFP、TH-Cre、TH-BAC-Cre和DAT-Cre小鼠品系视网膜中的转基因表达异质性。
Neuroscience. 2015 Oct 29;307:319-37. doi: 10.1016/j.neuroscience.2015.08.060. Epub 2015 Aug 31.
一种用于整个小鼠大脑的强大且高通量的 Cre 报告和表征系统。
Nat Neurosci. 2010 Jan;13(1):133-40. doi: 10.1038/nn.2467. Epub 2009 Dec 20.
4
A global double-fluorescent Cre reporter mouse.一种全球双荧光Cre报告基因小鼠。
Genesis. 2007 Sep;45(9):593-605. doi: 10.1002/dvg.20335.
5
Long-term depression: multiple forms and implications for brain function.长期抑郁：多种形式及其对脑功能的影响
Trends Neurosci. 2007 Apr;30(4):176-84. doi: 10.1016/j.tins.2007.02.005. Epub 2007 Mar 1.
6
Inducible Cre recombinase activity in mouse cerebellar granule cell precursors and inner ear hair cells.小鼠小脑颗粒细胞前体和内耳毛细胞中的诱导型Cre重组酶活性。
Dev Dyn. 2006 Nov;235(11):2991-8. doi: 10.1002/dvdy.20948.
7
Neuron-specific enolase-cre mouse line with cre activity in specific neuronal populations.在特定神经元群体中具有cre活性的神经元特异性烯醇化酶-cre小鼠品系。
Genesis. 2006 Mar;44(3):130-5. doi: 10.1002/gene.20197.
8
Math1 is expressed in temporally discrete pools of cerebellar rhombic-lip neural progenitors.Math1在小脑菱唇神经祖细胞的时间离散池中表达。
Neuron. 2005 Oct 6;48(1):17-24. doi: 10.1016/j.neuron.2005.08.028.
9
Morphogenetic and cellular movements that shape the mouse cerebellum; insights from genetic fate mapping.塑造小鼠小脑的形态发生和细胞运动；遗传命运图谱的见解
Neuron. 2005 Jan 6;45(1):27-40. doi: 10.1016/j.neuron.2004.12.021.
10
Disorders of the cerebellum: ataxia, dysmetria of thought, and the cerebellar cognitive affective syndrome.小脑疾病：共济失调、思维辨距障碍和小脑认知情感综合征。
J Neuropsychiatry Clin Neurosci. 2004 Summer;16(3):367-78. doi: 10.1176/jnp.16.3.367.