Yulia Zubova, Diana Nasyrova, Anna Sapronova, Michael Ugrumov
Laboratory of Neural and Neuroendocrine Regulations, Institute of Developmental Biology, Russian Academy of Sciences, 26 Vavilov str., 119334 Moscow, Russia.
Laboratory of Neural and Neuroendocrine Regulations, Institute of Developmental Biology, Russian Academy of Sciences, 26 Vavilov str., 119334 Moscow, Russia.
Mol Cell Endocrinol. 2014 Aug 5;393(1-2):92-8. doi: 10.1016/j.mce.2014.06.006. Epub 2014 Jun 18.
This study was aimed to test the authors' hypothesis stating that the developing brain before the closure of the blood brain barrier (BBB) operates as an endocrine organ that secretes classical neurotransmitters and neuropeptides into the general circulation. 5-Hydroxytryptamine (5-HT) was selected as a marker of brain endocrine activity though it is also secreted by peripheral organs. 5-HT was detected in blood of rats in a biologically active concentration at any studied age, from the 21st embryonic day till the 30th postnatal day. The brain was proven to be a source of circulating 5-HT before the BBB closure by showing that the 5-HT concentration in blood decreased significantly after the inhibition of 5-HT synthesis in the brain of neonates. The 5-HT concentration in blood was not diminished after the BBB closure, apparently due to compensatory increase of 5-HT secretion by peripheral sources. Thus, brain-derived 5-HT is delivered to the general circulation before the BBB closure being potentially capable of providing endocrine regulation of target organs.
本研究旨在验证作者的假设，即血脑屏障（BBB）闭合之前发育中的大脑作为一个内分泌器官，将经典神经递质和神经肽分泌到体循环中。5-羟色胺（5-HT）虽也由外周器官分泌，但被选为脑内分泌活动的标志物。在从胚胎第21天到出生后第30天的任何研究年龄阶段，均在大鼠血液中检测到具有生物活性浓度的5-HT。通过表明新生大鼠脑内5-HT合成受到抑制后血液中5-HT浓度显著降低，证明大脑是BBB闭合之前循环中5-HT的来源。BBB闭合后血液中5-HT浓度未降低，显然是由于外周来源5-HT分泌的代偿性增加。因此，脑源性5-HT在BBB闭合之前被输送到体循环中，有可能对靶器官进行内分泌调节。
