Edström S, Westin T, Delle U, Lundholm K
Department of Otolaryngology, Sahlgrenska Hospital, University of Gothenburg, Sweden.
Eur J Cancer Clin Oncol. 1989 Feb;25(2):227-32. doi: 10.1016/0277-5379(89)90013-8.
Flow cytometric DNA distribution and the activity of the enzyme ornithine decarboxylase (ODC) have been measured in tumor biopsies from patients with malignant head and neck tumors before and after 6-8 days on nasogastric tube feeding. Thirteen patients were randomized to the study group (defined enteral nutrition), and 13 patients to the control group (spontaneous oral intake). The relative size of the aneuploidic compartment was significantly increased in tumor biopsies from patients on enteral nutrition compared to controls on ad libitum oral intake. The aneuploidic compartment and DNA index were unrelated to histologic differentiation and to 1 year patient survival. Poorly differentiated tumors had higher ODC activity than moderately to highly differentiated tumors. ODC activity was positively correlated (r = 0.63, P less than 0.01) to the aneuploidic compartment size in tumors. Patients with less than 1 year survival and T4 tumors had a trend to higher (P less than 0.05) ODC activity compared to those with more than 1 year survival. In conclusion, this study demonstrates that nutritional support can change DNA distribution in human cancer. Such changes may either be related to activation of cell proliferation or tumor dedifferentiation.
在恶性头颈部肿瘤患者的肿瘤活检组织中，测量了鼻饲6 - 8天前后的流式细胞术DNA分布及鸟氨酸脱羧酶（ODC）活性。13例患者被随机分为研究组（确定的肠内营养），13例患者为对照组（自主经口摄入）。与自由经口摄入的对照组相比，接受肠内营养患者的肿瘤活检组织中非整倍体区室的相对大小显著增加。非整倍体区室和DNA指数与组织学分化及患者1年生存率无关。低分化肿瘤的ODC活性高于中高分化肿瘤。肿瘤中的ODC活性与非整倍体区室大小呈正相关（r = 0.63，P < 0.01）。与生存超过1年的患者相比，生存不足1年的患者及T4期肿瘤患者的ODC活性有升高趋势（P < 0.05）。总之，本研究表明营养支持可改变人类癌症中的DNA分布。此类改变可能与细胞增殖激活或肿瘤去分化有关。
