Westin T, Stein H, Niedobitek G, Lundholm K, Edström S
Department of Otolaryngology, Sahlgrenska Hospital, University of Göteborg, Sweden.
Am J Clin Nutr. 1991 Mar;53(3):764-8. doi: 10.1093/ajcn/53.3.764.
Refeeding of patients with malignant tumors may induce tumor-cell DNA synthesis. The present study was aimed at evaluating whether induction of altered cell-cycle kinetics could be induced by intravenous total parenteral nutrition (TPN) in tumor biopsies from head and neck cancers. Nine malnourished patients with squamous cell carcinoma in the head-and-neck area were investigated before and after 5-7 d of continuous TPN. Tumor biopsies were taken in both fasted and fed states for determination of 1) ornithine decarboxylase (ODC) activity, which is rate limiting for polyamine synthesis; 2) flow-cytometric-DNA-distribution measurements; and 3) the fraction of proliferating cells expressed as immunohistochemical reactivity with the monoclonal antibody Ki-67. The histopathological differentiation, the fraction of aneuploidic cells, ODC activity, and Ki-67 reactivity were not significantly related to each other, although the number of aneuploidic cells in replicative phases correlated with the number of cells expressing the Ki-67 antigen (r = 0.86, P less than 0.01). Tumor cytokinetics showed no evidence of being changed by TPN administration.
对恶性肿瘤患者进行再喂养可能会诱导肿瘤细胞的DNA合成。本研究旨在评估静脉全胃肠外营养（TPN）是否能诱导头颈部癌肿瘤活检组织中细胞周期动力学改变。对9例头颈部鳞状细胞癌营养不良患者在持续TPN 5 - 7天前后进行了研究。在禁食和进食状态下均取肿瘤活检组织，以测定：1）鸟氨酸脱羧酶（ODC）活性，其是多胺合成的限速酶；2）流式细胞术DNA分布测量；3）以与单克隆抗体Ki-67的免疫组化反应性表示的增殖细胞分数。组织病理学分化、非整倍体细胞分数、ODC活性和Ki-67反应性之间无显著相关性，尽管复制期非整倍体细胞数量与表达Ki-67抗原的细胞数量相关（r = 0.86，P < 0.01）。肿瘤细胞动力学未显示出TPN给药导致改变的证据。
