类风湿关节炎患者滑液中 CCR7-CD45RA-CD27+CD28+效应记忆亚群中 CD45RA-Foxp3(low) 非调节性 T 细胞增加。
CD45RA-Foxp3(low) non-regulatory T cells in the CCR7-CD45RA-CD27+CD28+ effector memory subset are increased in synovial fluid from patients with rheumatoid arthritis.
机构信息
Department of Rheumatology and Clinical Immunology, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan; Department of Evidence-Based Laboratory Medicine, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.
Department of Rheumatology and Clinical Immunology, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan; Department of Evidence-Based Laboratory Medicine, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.
出版信息
Cell Immunol. 2014 Jul;290(1):96-101. doi: 10.1016/j.cellimm.2014.05.011. Epub 2014 Jun 6.
Increased numbers of regulatory T (Treg) cells are found in synovial fluid from patients with rheumatoid arthritis (RASF) compared with peripheral blood. However, Treg cells in RASF have been shown to have a decreased capacity to suppress T cells. Here we phenotypically classified CD4+ T cells in RASF into six subsets based on the expression of CD45RA, CCR7, CD27 and CD28, and demonstrated that the CCR7-CD45RA-CD27+CD28+ TEM subset was significantly increased in synovial fluid compared with peripheral blood. In addition, the proportion of Foxp3+ Treg cells in the CCR7-CD45RA-CD27+CD28+ TEM subset was significantly increased in RASF. Furthermore, most of the Foxp3+ Treg cells in RASF were non-suppressive CD45RA-Foxp3(low) non-Treg cells, and the frequency of the non-Treg cells in the CCR7-CD45RA-CD27+CD28+ TEM subset was significantly increased in RASF. Our findings suggest that the pro-inflammatory environment in RA joints may induce the increase of CD45RA-Foxp3(low) non-Treg cells in synovial fluid.
在类风湿关节炎（RASF）患者的滑液中发现调节性 T（Treg）细胞数量增加，而外周血中则较少。然而，已经表明 RASF 中的 Treg 细胞抑制 T 细胞的能力降低。在这里，我们根据 CD45RA、CCR7、CD27 和 CD28 的表达，将 RASF 中的 CD4+ T 细胞表型分类为六个亚群，并证明 CCR7-CD45RA-CD27+CD28+ TEM 亚群在滑液中明显高于外周血。此外，在 RASF 中，CCR7-CD45RA-CD27+CD28+ TEM 亚群中的 Foxp3+ Treg 细胞比例明显增加。此外，RASF 中的大多数 Foxp3+ Treg 细胞是非抑制性的 CD45RA-Foxp3(low)非 Treg 细胞，并且在 CCR7-CD45RA-CD27+CD28+ TEM 亚群中的非 Treg 细胞频率在 RASF 中明显增加。我们的研究结果表明，RA 关节中的促炎环境可能诱导滑膜中 CD45RA-Foxp3(low)非 Treg 细胞的增加。
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