Tomiyama Hiroko, Takata Hiroshi, Matsuda Tomoko, Takiguchi Masafumi
Division of Viral Immunology, Center for AIDS Research, Kumamoto University, Honjo, Kumamoto, Japan.
Eur J Immunol. 2004 Apr;34(4):999-1010. doi: 10.1002/eji.200324478.
Phenotypic classification of human CD8(+) T cells using three cell surface markers, CD27, CD28 and CD45RA, was recently suggested to be useful for identification of naive, memory and effector CD8(+) T cells. However, it still remains unclear whether such classification precisely reflects functional classification of CD8(+) T cells. To clarify this, we characterized each CD27CD28CD45RA subset of total and human cytomegalovirus (HCMV)-specific CD8(+) T cells by analyzing the expression of perforin and two chemokine receptors, CCR5 and CCR7, as well as their function. An inverse correlation between perforin and CD27 expression was found in all four CD28CD45RA subsets. Therefore, to achieve a phenotypic classification of CD8(+) T cells that more precisely reflects their function, the CD27(+) subset was divided into CD27(low) and CD27(high) subsets based on the expression level of CD27. Functional and flow cytometric analyses of CD27CD28CD45RA subsets showed that this phenotypic classification reflects functional classification of CD8(+) T cells. HCMV-specific CD8(+) T cells from healthy HCMV-seropositive individuals were predominantly found in effector and memory/effector subsets, indicating that HCMV-specific effector CD8(+) T cells are actively induced by HCMV replication in healthy HCMV carriers. Phenotypic analyses of CD8(+) T cells using this classification will enable the characterization of antigen-specific CD8(+) T cells.
最近有人提出，使用三种细胞表面标志物CD27、CD28和CD45RA对人CD8(+) T细胞进行表型分类，有助于识别初始、记忆和效应CD8(+) T细胞。然而，这种分类是否能准确反映CD8(+) T细胞的功能分类仍不清楚。为了阐明这一点，我们通过分析穿孔素和两种趋化因子受体CCR5和CCR7的表达及其功能，对总CD8(+) T细胞和人巨细胞病毒（HCMV）特异性CD8(+) T细胞的每个CD27CD28CD45RA亚群进行了表征。在所有四个CD28CD45RA亚群中，均发现穿孔素与CD27表达呈负相关。因此，为了实现能更准确反映其功能的CD8(+) T细胞表型分类，根据CD27的表达水平，将CD27(+)亚群分为CD27(low)和CD27(high)亚群。对CD27CD28CD45RA亚群的功能和流式细胞术分析表明，这种表型分类反映了CD8(+) T细胞的功能分类。健康的HCMV血清阳性个体的HCMV特异性CD8(+) T细胞主要存在于效应和记忆/效应亚群中，这表明在健康的HCMV携带者中，HCMV复制可积极诱导HCMV特异性效应CD8(+) T细胞。使用这种分类对CD8(+) T细胞进行表型分析，将有助于对抗原特异性CD8(+) T细胞进行表征。
