Stratford I J, Stephens M A
MRC Radiobiology Unit, Didcot, Oxon, U.K.
Int J Radiat Oncol Biol Phys. 1989 Apr;16(4):973-6. doi: 10.1016/0360-3016(89)90898-5.
We obtained good agreement between the MTT assay and conventional clonogenic assays regarding the concentration and contact time required to produce a given level of killing of Chinese hamster V79 cells treated in either air of N2 with a range of bioreductive cytotoxic drugs. All agents chosen for these experiments represented classes of compounds known to be more toxic towards hypoxic cells than they are to aerobic cells. Namely a quinone, mitomycin C; a di-N-oxide, SR4233; and a number of nitro-heterocyclics including misonidazole. The MTT assay is carried out with V79 cells attached to the bottom of 1 cm glass wells within a 24 well plate. All procedures, that is drug exposure, cell growth, metabolism of MTT are then carried out in situ. To measure optical density we used an ELIZA plate reader modified to take 24-well plates. We propose that this method provides a simple, rapid procedure for evaluating the cytotoxicity of bioreductive drugs.
我们发现，在使用一系列生物还原细胞毒性药物处理中国仓鼠V79细胞时，MTT法与传统克隆形成试验在产生给定杀伤水平所需的浓度和接触时间方面取得了良好的一致性。这些实验中选择的所有药物均代表已知对缺氧细胞比对有氧细胞毒性更大的化合物类别。具体来说，有醌类的丝裂霉素C；二N-氧化物SR4233；以及多种硝基杂环化合物，包括米索硝唑。MTT试验是在24孔板中1厘米玻璃孔底部附着的V79细胞上进行的。然后所有步骤，即药物暴露、细胞生长、MTT代谢均在原位进行。为了测量光密度，我们使用了经过改装以读取24孔板的酶标仪。我们认为该方法为评估生物还原药物的细胞毒性提供了一种简单、快速的程序。
