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通过具有交错可变密度采集加权采样模式的间接¹³C化学位移成像追踪植物中的代谢物动态。

Tracking metabolite dynamics in plants via indirect 13C chemical shift imaging with an interleaved variable density acquisition weighted sampling pattern.

作者信息

Fuchs Johannes, Melkus Gerd, Borisjuk Ljudmilla, Jakob Peter

机构信息

Department of Experimental Physics 5 (Biophysics), University of Würzburg, Würzburg, Germany,

出版信息

MAGMA. 2015 Apr;28(2):127-34. doi: 10.1007/s10334-014-0453-4. Epub 2014 Jun 22.

DOI:10.1007/s10334-014-0453-4
PMID:24952645
Abstract

OBJECTIVE

Developing and evaluating an improved sampling pattern to track the dynamics of labeled substances in plants using indirect (13)C chemical shift imaging.

MATERIALS AND METHODS

An algorithm to split an acquisition weighted sampling pattern into several undersampled sub-images is presented. The sampling patterns are used in CSI moving phantom experiments as well as in in vivo POCE-CSI experiments on barley stem and grain. Reconstruction is performed traditionally or by compressed sensing.

RESULTS

The moving phantom experiments show that the sampling pattern can reduce motion artifacts at the cost of an increased overall noise. The in vivo experiments demonstrate the feasibility of extracting a time series from a single imaging experiment.

CONCLUSION

The sampling pattern is suitable for tracking the uptake of label substances into plant material. The use of compressed sensing allows an increased spatial and temporal resolution.

摘要

目的

开发并评估一种改进的采样模式,以利用间接(13)C化学位移成像追踪植物中标记物质的动态变化。

材料与方法

提出一种将采集加权采样模式分割成多个欠采样子图像的算法。该采样模式用于CSI移动模型实验以及大麦茎和籽粒的体内POCE-CSI实验。重建采用传统方法或压缩感知方法。

结果

移动模型实验表明,该采样模式可以减少运动伪影,但代价是整体噪声增加。体内实验证明了从单个成像实验中提取时间序列的可行性。

结论

该采样模式适用于追踪标记物质进入植物材料的摄取过程。压缩感知的使用可提高空间和时间分辨率。

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Plant Cell. 2011 Aug;23(8):3041-54. doi: 10.1105/tpc.111.087015. Epub 2011 Aug 19.
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Dynamic ¹³C/¹ H NMR imaging uncovers sugar allocation in the living seed.
动态 ¹³C/¹ H NMR 成像揭示活体种子中的糖分配。
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Interleaved variable density sampling with a constrained parallel imaging reconstruction for dynamic contrast-enhanced MR angiography.交错变密度采样结合约束并行成像重建在动态对比增强磁共振血管成像中的应用。
Magn Reson Med. 2011 Aug;66(2):428-36. doi: 10.1002/mrm.22814. Epub 2011 Feb 28.
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Barley grain development toward an integrative view.大麦籽粒发育的综合观点。
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