Casartelli-Alves L, Boechat V C, Macedo-Couto R, Ferreira L C, Nicolau J L, Neves L B, Millar P R, Vicente R T, Oliveira R V C, Muniz A G, Bonna I C F, Amendoeira M R R, Silva R C, Langoni H, Schubach T M P, Menezes R C
Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Av. Brasil, 4365, Rio de Janeiro, RJ CEP: 21040-360, Brazil.
Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil, 4365, Rio de Janeiro, RJ CEP: 21040-360, Brazil.
Vet Parasitol. 2014 Aug 29;204(3-4):346-51. doi: 10.1016/j.vetpar.2014.05.039. Epub 2014 Jun 4.
Since free-range chickens are important for the epidemiology of toxoplasmosis, this study evaluated the sensitivity and specificity of different laboratory techniques for the diagnosis of Toxoplasma gondii in these animals. Serum samples from 135 adult domestic chickens were tested for anti-T. gondii antibodies by the indirect fluorescent antibody test (IFAT), enzyme-linked immunosorbent assay (ELISA), modified agglutination test (MAT), and indirect hemagglutination test (IHAT). Tissue samples from all animals were analyzed by histopathology, immunohistochemistry and mouse bioassay (gold standard). Fifty-four chickens were positive for T. gondii in the bioassay. The sensitivity and specificity of the different tests were, respectively, 85% and 56% for ELISA; 80% and 52% for IFAT; 76% and 68% for MAT; 61% and 80% for IHAT; 7% and 98% for immunohistochemistry, and 6% and 98% for histopathology. The MAT was the most effective method for the diagnosis of T. gondii infection in chickens, followed by ELISA. Histopathology and immunohistochemistry are useful tools for the diagnosis of T. gondii infection in chickens due to their specificity.
由于散养鸡对弓形虫病的流行病学具有重要意义，本研究评估了不同实验室技术对这些动物弓形虫诊断的敏感性和特异性。对135只成年家鸡的血清样本进行检测，采用间接荧光抗体试验（IFAT）、酶联免疫吸附测定（ELISA）、改良凝集试验（MAT）和间接血凝试验（IHAT）检测抗弓形虫抗体。对所有动物的组织样本进行组织病理学、免疫组织化学和小鼠生物测定（金标准）分析。生物测定中有54只鸡弓形虫呈阳性。不同检测方法的敏感性和特异性分别为：ELISA为85%和56%；IFAT为80%和52%；MAT为76%和68%；IHAT为61%和80%；免疫组织化学为7%和98%，组织病理学为6%和98%。MAT是诊断鸡弓形虫感染最有效的方法，其次是ELISA。组织病理学和免疫组织化学因其特异性，是诊断鸡弓形虫感染的有用工具。
