巴西刺豚鼠中弓形虫抗体的出现：诊断技术比较

Occurrence of Toxoplasma gondii antibodies in Dasyprocta aguti from Brazil: comparison of diagnostic techniques.

作者信息

Soares Herbert S, Minervino Antonio H H, Barrêto-Júnior Raimundo A, Neves Kedson A L, Oliveira Moacir F, Santos Jackelyne R, van Sauers Astrid R, Dubey Jitender P, Gennari Solange M

机构信息

Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, 05508-270, Cidade Universitária, São Paulo/SP, Brazil.

出版信息

J Zoo Wildl Med. 2011 Dec;42(4):763-5. doi: 10.1638/2011-0136.1.

DOI:10.1638/2011-0136.1

PMID:22204080

Abstract

In this study, the occurrence of antibodies to Toxoplasma gondii in Brazilian agouti (Dasyprocta aguti) was compared by modified agglutination test (MAT) and indirect fluorescent antibody test (IFAT) using anti-capybara conjugate. Sera from 109 animals were tested using MAT (1:25 cut-off) and IFAT (1:16 cut-off); 19% were positive by MAT, and 18% were positive by IFAT. Overall, the 17 IFAT-positive samples were also positive for MAT. The four positive MAT samples with a titer < or = 200 were IFAT negative. All negative samples obtained by MAT matched with the results of the IFAT. Comparing both tests, and considering MAT as the gold standard, the sensitivity of IFAT was 81%, the specificity was 100%, the accuracy was 97%, the positive predictive value (PPV) was 100%, and the negative predictive value 96%. The kappa value agreement was 87.3% (75.1-99.6%). The anti-capybara conjugate can be successfully used to perform IFAT in Brazilian agouti with maximum specificity and PPV.

摘要

在本研究中，使用抗水豚结合物，通过改良凝集试验（MAT）和间接荧光抗体试验（IFAT）比较了巴西刺豚鼠（Dasyprocta aguti）中弓形虫抗体的出现情况。使用MAT（截断值1:25）和IFAT（截断值1:16）对109只动物的血清进行检测；MAT检测阳性率为19%，IFAT检测阳性率为18%。总体而言，17份IFAT检测阳性的样本MAT检测也呈阳性。MAT检测中滴度≤200的4份阳性样本IFAT检测为阴性。MAT检测获得的所有阴性样本与IFAT检测结果相符。将两种检测方法进行比较，并将MAT作为金标准，IFAT的灵敏度为81%，特异性为100%，准确性为97%，阳性预测值（PPV）为100%，阴性预测值为96%。kappa值一致性为87.3%（75.1 - 99.6%）。抗水豚结合物可成功用于对巴西刺豚鼠进行IFAT检测，具有最高的特异性和PPV。