Galat Maryna, Moré Gaston, Frey Caroline F, Kovalenko Ganna, Maliuk Inna, Halka Ihor, Sytiuk Mykola, Bezymennyi Maksym, Galat Vladyslav, Jokelainen Pikka
Institute of Parasitology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty University of Bern, Länggassstrasse 122, 3012, Bern, Switzerland.
Faculty of Veterinary Medicine, National University of Life and Environmental Sciences of Ukraine, Heroiv Oborony Str. 15, 03041, Kyiv, Ukraine.
Int J Parasitol Parasites Wildl. 2023 Dec 29;23:100901. doi: 10.1016/j.ijppaw.2023.100901. eCollection 2024 Apr.
is an important zoonotic parasite worldwide, but it has received limited attention in Ukraine. A seroepidemiological study was conducted and samples from 452 wild boars that had been hunted in 2006-2011 in 23 of the 25 regions of Ukraine were tested to estimate . seroprevalence. A locally available commercial enzyme-linked immunosorbent assay (ELISA) was used for the investigation. Additionally, we tested 92 of the sera using a widely used commercial multi-species ELISA and an indirect immunofluorescence antibody test (IFAT). With the locally available ELISA, 35 of the 452 wild boars tested positive, yielding a seroprevalence estimate of 7.7% (95% confidence interval 5.5-10.5). The seropositive wild boars originated from eight of the regions. Using the majority criteria, 10/92 samples tested using both ELISAs and the IFAT were considered positive, yielding an estimated seroprevalence of 10.9% within the subset of samples. The highest seroprevalence was observed in wild boars hunted in Luhans'k (30.0%), Odesa (17.7%) and Kharkiv (12.7%). Seroprevalence was higher in older animals (13.3% for age group >12 months and 7.7% for age group ≤12 months). This is the first seroepidemiological study of in wild boars in Ukraine. Assuming that seropositivity indicates presence of infectious parasites in the tissues, eating undercooked meat of wild boars hunted in Ukraine could be a potential source of infection to other hosts, including humans.
是一种在全球范围内重要的人畜共患寄生虫,但在乌克兰受到的关注有限。开展了一项血清流行病学研究,对2006 - 2011年在乌克兰25个地区中的23个地区猎杀的452头野猪的样本进行检测,以估计血清阳性率。使用当地可得的商业酶联免疫吸附测定(ELISA)进行调查。此外,我们使用一种广泛使用的商业多物种ELISA和间接免疫荧光抗体试验(IFAT)对92份血清进行了检测。使用当地可得的ELISA,452头野猪中有35头检测呈阳性,血清阳性率估计为7.7%(95%置信区间5.5 - 10.5)。血清阳性的野猪来自8个地区。根据多数标准,在同时使用两种ELISA和IFAT检测的92份样本中,10份被认为呈阳性,在样本子集中估计血清阳性率为10.9%。在卢甘斯克(30.0%)、敖德萨(17.7%)和哈尔科夫(12.7%)猎杀的野猪中观察到最高的血清阳性率。老年动物的血清阳性率更高(年龄组>12个月为13.3%,年龄组≤12个月为7.7%)。这是乌克兰首次对野猪进行的血清流行病学研究。假设血清阳性表明组织中存在感染性寄生虫,食用在乌克兰猎杀的野猪的未煮熟肉可能是包括人类在内的其他宿主的潜在感染源。