Rakic L M, Zlokovic B V, Segal M B, Lipovac M H, Mitrovic D M, Veskov R, Mackic J B, Davson H
Institute of Biochemistry, School of Medicine Belgrade, Yugoslavia.
Metab Brain Dis. 1989 Mar;4(1):9-15. doi: 10.1007/BF00999488.
Effects of sensory-motor cortical lesions on the function of the blood-brain barrier in distant brain areas are poorly understood. Therefore a brain vascular perfusion method has been used to measure simultaneously the kinetics of entry of two inert polar molecules, D-[14C]mannitol (MW 180) and [3H]polyethylene glycol (PEG; MW 4000), into the parietal cortex, hippocampus, and caudate nucleus in guinea pigs with ipsilateral and contralateral sensory-motor cortical lesions. The graphically determined cerebral capillary unidirectional constant, Kin, indicated a marked increase in blood-to-brain transport of both molecules in all regions studied, the changes being significantly higher after contralateral lesion. The mannitol/PEG cerebrovascular permeability constant ratio, Pman/PPEG, suggested the opening up of channels that permit a flow of fluid carrying substances either with respect to (2 days after ipsilateral lesion) or irrespective of their molecular size, depending on the time after lesion. Amphetamine treatment in the guinea pigs with sensory-motor lesions induced more pronounced blood-brain barrier permeability changes for both molecules in distant brain areas.
感觉运动皮层损伤对远隔脑区血脑屏障功能的影响尚不清楚。因此，采用一种脑血管灌注方法，同时测量D-[14C]甘露醇（分子量180）和[3H]聚乙二醇（PEG；分子量4000）这两种惰性极性分子进入患有同侧和对侧感觉运动皮层损伤的豚鼠顶叶皮层、海马和尾状核的动力学。通过图形确定的脑毛细血管单向常数Kin表明，在所有研究区域中，这两种分子的血脑转运均显著增加，对侧损伤后的变化明显更高。甘露醇/PEG脑血管通透性常数比值Pman/PPEG表明，根据损伤后的时间，会开放允许携带物质的流体流动的通道，这些通道与物质的分子大小有关（同侧损伤后2天）或无关。对患有感觉运动损伤的豚鼠进行苯丙胺治疗，会在远隔脑区引起这两种分子更明显的血脑屏障通透性变化。
