Zlokovic B V, Skundric D S, Segal M B, Colover J, Jankov R M, Pejnovic N, Lackovic V, Mackic J, Lipovac M N, Davson H
Institute of Physiology, Medical Faculty, University of Belgrade, Yugoslavia.
Metab Brain Dis. 1989 Mar;4(1):33-40. doi: 10.1007/BF00999491.
Blood-brain barrier permeability to homologous serum 125I-IgG and to D-[3H]mannitol was studied by means of the brain vascular perfusion method in guinea pigs with experimental allergic encephalomyelitis (EAE). EAE was induced with homologous myelin basic protein (MBP) after pretreatment with foreign protein and muramyl dipeptide (MDP). The results suggest a significant comparable increase in IgG blood-to-brain clearance in the parietal cortex, hippocampus, and caudate nucleus, during vascular perfusion of the brains of animals, after 7 and 20 days of EAE. On the other hand, unidirectional transfer of mannitol in the same period of EAE was markedly augmented only in the hippocampus, but no significant changes in the parietal cortex or caudate nucleus were observed. Cerebrospinal fluid (CSF)/serum ratios for IgG and albumin were both significantly increased, suggesting an increase in blood-CSF barrier permeability, but more for albumin than for IgG. The results were confirmed by immunohistochemical determination of the IgG deposits in the brains of EAE animals, during vascular perfusion with unlabeled homologous IgG. An important role of the blood-brain barrier for the central nervous system immunoglobulin homeostasis during EAE is suggested.
采用脑血管灌注法，对患有实验性变应性脑脊髓炎（EAE）的豚鼠血脑屏障对同源血清125I-IgG和D-[3H]甘露醇的通透性进行了研究。在用异种蛋白和胞壁酰二肽（MDP）预处理后，用同源髓鞘碱性蛋白（MBP）诱导EAE。结果表明，在EAE发病7天和20天后，对动物脑进行血管灌注时，顶叶皮质、海马和尾状核中IgG的血脑清除率显著增加且具有可比性。另一方面，在EAE的同一时期，甘露醇的单向转运仅在海马中显著增强，而在顶叶皮质或尾状核中未观察到显著变化。脑脊液（CSF）/血清中IgG和白蛋白的比值均显著升高，表明血脑脊液屏障通透性增加，但白蛋白的增加幅度大于IgG。在用未标记的同源IgG进行血管灌注期间，通过免疫组织化学测定EAE动物脑中IgG沉积物，证实了上述结果。提示血脑屏障在EAE期间对中枢神经系统免疫球蛋白稳态起重要作用。
