Mimmo Tanja, Bartucca Maria Luce, Del Buono Daniele, Cesco Stefano
Faculty of Science and Technology, Free University of Bolzano, Piazza Università 5, 39100 Bolzano, Italy.
Dipartimento di Scienze Agrarie, Alimentari e Ambientali, Università degli Studi di Perugia, Borgo XX Giugno 74, 06121 Perugia, Italy.
Chemosphere. 2015 Jan;119:31-36. doi: 10.1016/j.chemosphere.2014.04.114. Epub 2014 Jun 20.
The phytoextraction capacity of Italian ryegrass (Lolium multiflorum L.) to remove terbuthylazine (TBA) from aqueous solution has been assessed using a plant-based biotest (RHIZOtest). Three TBA concentrations (0.5, 1.0 and 2.0 mg L(-1)) were chosen to evaluate the tolerance capacity of the ryegrass. Even though the treatments negatively affected plants, they were able to remove up to 30-40% of TBA. In addition, some enzymatic activities involved in the response to TBA-induced stress were determined. Glutathione S-transferase (GST) has been activated with a TBA-dose dependent trend; ascorbate peroxidase (APX) activities have been induced within the first hours after the treatments, followed by decreases or disappearance in plants exposed to two higher dosages. In conclusion, this case-study highlights that the combination of ryegrass and RHIZOtest resulted to be effective in the remediation of aqueous solutions polluted by TBA.
利用基于植物的生物测试（RHIZOtest）评估了多花黑麦草（Lolium multiflorum L.）从水溶液中去除特丁津（TBA）的植物提取能力。选择了三种TBA浓度（0.5、1.0和2.0 mg L(-1)）来评估黑麦草的耐受能力。尽管这些处理对植物有负面影响，但它们能够去除高达30%-40%的TBA。此外，还测定了一些与TBA诱导的胁迫反应相关的酶活性。谷胱甘肽S-转移酶（GST）以TBA剂量依赖性趋势被激活；抗坏血酸过氧化物酶（APX）活性在处理后的最初几个小时内被诱导，随后在暴露于两种较高剂量的植物中降低或消失。总之，这个案例研究强调了黑麦草和RHIZOtest的组合在修复受TBA污染的水溶液方面是有效的。
