Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Zatisi 728/II, 389 25, Vodnany, Czech Republic.
Environ Sci Pollut Res Int. 2016 Dec;23(23):24289-24297. doi: 10.1007/s11356-016-7689-7. Epub 2016 Sep 21.
The sensitivity of early life stages of common carp (Cyprinus carpio L.) to chronic exposure to single and combined environmental concentrations of the triazine metabolites terbuthylazine 2-hydroxy, terbuthylazine-desethyl and atrazine 2-hydroxy was evaluated under laboratory conditions. Their effects were assessed on lipid peroxidation, antioxidant enzymes (total superoxide dismutase, glutathione reductase, catalase, glutathione S-transferase, reduced glutathione), mortality, growth, development and histology. Single metabolites (terbuthylazine 2-hydroxy-0.73 μg/L; terbuthylazine-desethyl-1.80 μg/L; atrazine 2-hydroxy-0.66 μg/L) and combinations were not associated with negative effects on hatching, behaviour, embryo viability, growth or early ontogeny. Carp exposed to terbuthylazine-desethyl at 1.80 μg/L showed significantly lower total superoxide dismutase and glutathione reductase activity compared with the control group. Liver histology revealed diffused steatosis associated with the presence of lipid inclusions in hepatic cells in groups exposed to terbuthylazine-desethyl, atrazine 2-hydroxy and the tested combination of metabolites.
实验室条件下评估了鲤鱼（Cyprinus carpio L.）早期生活阶段对单一和组合环境浓度的三嗪代谢物特丁津 2-羟、特丁津去乙基和阿特拉津 2-羟的慢性暴露的敏感性。评估了它们对脂质过氧化、抗氧化酶（总超氧化物歧化酶、谷胱甘肽还原酶、过氧化氢酶、谷胱甘肽 S-转移酶、还原型谷胱甘肽）、死亡率、生长、发育和组织学的影响。单一代谢物（特丁津 2-羟-0.73μg/L；特丁津去乙基-1.80μg/L；阿特拉津 2-羟-0.66μg/L）和组合对孵化、行为、胚胎活力、生长或早期发育均无不良影响。暴露于 1.80μg/L 特丁津去乙基的鲤鱼的总超氧化物歧化酶和谷胱甘肽还原酶活性明显低于对照组。肝组织学显示，暴露于特丁津去乙基、阿特拉津 2-羟和测试组合代谢物的组中，肝细胞内存在脂质包涵体，伴有弥漫性脂肪变性。
