Clin Chem Lab Med. 2014 Dec;52(12):1843-50. doi: 10.1515/cclm-2014-0487.
Aberrant expression of SRY-box containing gene 17 (SOX17) has been observed in several solid tumors. However, little is known about SOX17 expression in acute myeloid leukemia (AML). The purpose of this study was to investigate the alteration of SOX17 expression and to explore its clinical significance in AML.
Real-time quantitative PCR (RQ-PCR) was performed to analyze the status of SO1X17 expression in 103 patients with de novo AML and 26 normal controls. The clinical relevance of SOX17 expression was analyzed in AML.
SOX17 level in AML was significantly down-regulated compared to controls (p<0.001). Receiver operating characteristic curve (ROC) curve analysis revealed that an area under the ROC curve (AUC) of 0.834 (95% CI 0.765-0.903; p<0.0001) or 0.789 (95% CI 0.690-0.888, p<0.001) in discriminating all patients or cytogenetically normal patients from controls, respectively. The cohort of AML patients was divided into two groups according to the cut-off value of 0.017 (60% sensitivity and 100% specificity, respectively). Cytogenetically normal patients with low SOX17 expression had significantly shorter OS than those with high SOX17 expression (median 4 vs. 25 months, respectively, p=0.035). Multivariate analysis confirmed low SOX17 expression as an independent risk factor.
Our findings indicated that low SOX17 level may define an important risk factor in AML with normal cyotgenetics.
在几种实体肿瘤中观察到性决定区 Y 框蛋白 17（SOX17）的异常表达。然而，关于急性髓系白血病（AML）中 SOX17 表达的了解甚少。本研究旨在研究 SOX17 表达的改变，并探讨其在 AML 中的临床意义。
采用实时定量 PCR（RQ-PCR）分析 103 例初诊 AML 患者和 26 例正常对照者 SO1X17 表达情况。分析 SOX17 表达与 AML 临床相关性。
AML 中 SOX17 水平明显低于对照组（p<0.001）。ROC 曲线分析显示，曲线下面积（AUC）为 0.834（95%CI 0.765-0.903；p<0.0001）或 0.789（95%CI 0.690-0.888，p<0.001），可分别用于区分所有患者或细胞遗传学正常患者与对照组。根据 0.017 的截止值，将 AML 患者分为两组（分别为 60%的敏感性和 100%的特异性）。SOX17 表达水平低的细胞遗传学正常患者的 OS 明显短于 SOX17 表达水平高的患者（中位数分别为 4 个月和 25 个月，p=0.035）。多变量分析证实低 SOX17 表达是独立的危险因素。
我们的研究结果表明，SOX17 水平降低可能是细胞遗传学正常 AML 的一个重要危险因素。
