Sugiyama Akifumi, Ueda Yoshikatsu, Zushi Takahiro, Takase Hisabumi, Yazaki Kazufumi
Research Institute for Sustainable Humanosphere, Kyoto University, Uji, Kyoto, Japan.
Faculty of Bioenvironmental Science, Kyoto Gakuen University, Kameoka, Kyoto, Japan.
PLoS One. 2014 Jun 23;9(6):e100709. doi: 10.1371/journal.pone.0100709. eCollection 2014.
Highly diverse communities of bacteria inhabiting soybean rhizospheres play pivotal roles in plant growth and crop production; however, little is known about the changes that occur in these communities during growth. We used both culture-dependent physiological profiling and culture independent DNA-based approaches to characterize the bacterial communities of the soybean rhizosphere during growth in the field. The physiological properties of the bacterial communities were analyzed by a community-level substrate utilization assay with BioLog Eco plates, and the composition of the communities was assessed by gene pyrosequencing. Higher metabolic capabilities were found in rhizosphere soil than in bulk soil during all stages of the BioLog assay. Pyrosequencing analysis revealed that differences between the bacterial communities of rhizosphere and bulk soils at the phylum level; i.e., Proteobacteria were increased, while Acidobacteria and Firmicutes were decreased in rhizosphere soil during growth. Analysis of operational taxonomic units showed that the bacterial communities of the rhizosphere changed significantly during growth, with a higher abundance of potential plant growth promoting rhizobacteria, including Bacillus, Bradyrhizobium, and Rhizobium, in a stage-specific manner. These findings demonstrated that rhizosphere bacterial communities were changed during soybean growth in the field.
栖息于大豆根际的高度多样化细菌群落对植物生长和作物产量起着关键作用；然而，对于这些群落在生长过程中发生的变化却知之甚少。我们采用基于培养的生理特征分析和基于非培养的DNA方法，对田间生长的大豆根际细菌群落进行了特征描述。通过使用Biolog Eco平板进行群落水平的底物利用分析，分析了细菌群落的生理特性，并通过基因焦磷酸测序评估了群落的组成。在Biolog分析的所有阶段，根际土壤中的代谢能力均高于非根际土壤。焦磷酸测序分析揭示了根际和非根际土壤细菌群落在门水平上的差异；即在生长过程中，根际土壤中变形菌门增加，而酸杆菌门和厚壁菌门减少。对操作分类单元的分析表明，根际细菌群落在生长过程中发生了显著变化，包括芽孢杆菌属、慢生根瘤菌属和根瘤菌属在内的潜在植物促生根际细菌在特定阶段丰度更高。这些发现表明，在田间大豆生长过程中，根际细菌群落发生了变化。
