Li Lina, Huang Juying, Liu Yushan, Zhang Qian, Han Qingdian, Liu Yunguo, Zhang Guangna, Wang Xiao, Zhao Wenfei, Liu Lingxiao
College of Agriculture and Forestry, Linyi University, Linyi, 276000, China.
Shandong (Linyi) Institute of Modern Agriculture, Zhejiang University, Linyi, 276000, China.
Int Microbiol. 2025 Feb;28(2):277-288. doi: 10.1007/s10123-024-00542-6. Epub 2024 Jun 4.
Salvia miltiorrhiza is a kind of medicinal plant with various pharmacological activities. Few studies on the composition and diversity of rhizosphere microbial communities at different growth stages have been conducted on Salvia miltiorrhiz; in particular, salviorrhiza grows in soil that has been continuously planted for 3 years. The purpose of this study was to understand the changes of soil physicochemical properties of Salvia miltiorrhiza at different growth stages, and to study the composition and diversity of rhizosphere microbial community at different growth stages. Illumina NovaSeq sequencing technology was used to analyze the bacterial 16S rRNA gene and the fungal ITS region in the rhizosphere soil of Salvia miltiorrhiza at different growth stages. The results showed that the dominant bacterial phyla in the Salvia miltiorrhiza rhizosphere were Proteobacteria, Bacteroidetes, Acidobacteria, Firmicutes, Actinobacteria, and Chloroflexi. The dominant fungal phyla were Ascomycota, Mortierellomycota, Basidiomycota, and Rozellomycota. During the growth of Salvia miltiorrhiza, the physical and chemical properties of soil changed. As the Salvia miltiorrhiza grew, the content of available phosphorus, available potassium, pH, nitrate nitrogen, and ammonium nitrogen significantly decreased. Ammonium nitrogen and nitrate nitrogen had a greater impact on the bacterial community structure in the rhizosphere than on the fungal community structure. The work was to reveal differences in the rhizosphere bacterial and fungal community structure during different growth stages of Salvia miltiorrhiza, further understand the changes of rhizosphere microbial ecological characteristics and soil physicochemical properties during the cultivation of Salvia miltiorrhiza.
丹参是一种具有多种药理活性的药用植物。目前针对丹参不同生长阶段根际微生物群落的组成和多样性的研究较少；特别是在连续种植3年的土壤中生长的丹参。本研究的目的是了解丹参不同生长阶段土壤理化性质的变化，并研究不同生长阶段根际微生物群落的组成和多样性。采用Illumina NovaSeq测序技术分析丹参不同生长阶段根际土壤中的细菌16S rRNA基因和真菌ITS区域。结果表明，丹参根际的优势细菌门为变形菌门、拟杆菌门、酸杆菌门、厚壁菌门、放线菌门和绿弯菌门。优势真菌门为子囊菌门、被孢霉门、担子菌门和罗兹菌门。在丹参生长过程中，土壤理化性质发生了变化。随着丹参的生长，有效磷、有效钾、pH值、硝态氮和铵态氮的含量显著下降。铵态氮和硝态氮对根际细菌群落结构的影响大于对真菌群落结构的影响。这项工作旨在揭示丹参不同生长阶段根际细菌和真菌群落结构的差异，进一步了解丹参种植过程中根际微生物生态特征和土壤理化性质的变化。
