Zhang Min, Zhu Rongrong, Zhang Minfeng, Wang Shilong
Division of Cardiology, TongRen Hospital, Shanghai Jiao Tong University School of Medicine, 1111 Xianxia Road, Shanghai 200336, China.
School of Life Science and Technology, Tongji University, 1239 Siping Road, Shanghai 200092, China.
J Biosci Bioeng. 2014 Nov;118(5):508-13. doi: 10.1016/j.jbiosc.2014.04.016. Epub 2014 Jun 21.
Laser radiation is an efficient approach for rapid improvement of industrial microbial phenotypes. To improve ethanol tolerance in Saccharomyces cerevisiae strains, a 266 nm laser radiation with the use of repetitive cultivation was explored in this work. After irradiated by 266 nm laser radiation and repetitive cultivation, a genetically stable SM4 strain was obtained. The SM4 strain could grow on YPD plate with extra 15% (v/v) ethanol. Moreover, the ethanol production performance of SM4 strain was 29.25% more than that of the wild type strain when they were cultivated in 5% (v/v) ethanol fermentation medium for 72 h. The DNA mutation was the possible characters for the phenotype of SM4 strain. Overall, the 266 nm laser radiation and repetitive cultivation approach might be a novel and useful for breeding fermentation microorganisms.
激光辐射是一种快速改善工业微生物表型的有效方法。为提高酿酒酵母菌株的乙醇耐受性，本研究探索了使用重复培养的266nm激光辐射方法。经266nm激光辐射和重复培养后，获得了遗传稳定的SM4菌株。SM4菌株能够在添加额外15%（v/v）乙醇的YPD平板上生长。此外，当SM4菌株和野生型菌株在5%（v/v）乙醇发酵培养基中培养72小时时，SM4菌株的乙醇生产性能比野生型菌株高29.25%。DNA突变可能是SM4菌株表型的特征。总体而言，266nm激光辐射和重复培养方法可能是一种用于发酵微生物育种的新颖且有用的方法。
