Introduction of macromolecules into primary cultured neuronal cells by fusion with erythrocyte ghosts.
作者信息
Hikawa N, Horie H, Kawakami T, Okuda K, Takenaka T
机构信息
Department of Physiology, Yokohama City University School of Medicine, Japan.
出版信息
Brain Res. 1989 Feb 27;481(1):162-4. doi: 10.1016/0006-8993(89)90497-6.
PMID:2495838
Abstract
The macromolecules were injected into primary cultured neuronal cells by fusion with human erythrocyte ghosts containing macromolecules. The injection efficiency was about 30% and 80% of the cells extended neurites like normal neuronal cells. In order to succeed in this method, phytohaemagglutinin E and polyethylene glycol 1000 were essential, and monolayer culture cells were used.
摘要
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