Kriegler M P, Livingston D M
Somatic Cell Genet. 1977 Nov;3(6):603-10. doi: 10.1007/BF01539068.
Microinjection of physiologic quantities of macromolecules into tissue culture cells can facilitate the study of the biological effects of such macromolecules. In this communication, we describe a chemical technique which can be used to microinject proteins into monolayers of intact cells. Protein is loaded into erthrocyte ghosts, and the ghosts are then fused to the monolayer with polyethylene glycol 1000. Receipient cells can be injected with an efficiency of greater than 90% and contain an average of 3.8 X 10(6) microinjected molecules per cell. This technique circumvents certain problems encountered in virus-induced microinjection.
将生理量的大分子显微注射到组织培养细胞中，有助于研究此类大分子的生物学效应。在本报告中，我们描述了一种化学技术，可用于将蛋白质显微注射到完整细胞的单层中。将蛋白质加载到红细胞血影中，然后用聚乙二醇1000将血影与单层细胞融合。受体细胞的注射效率大于90%，每个细胞平均含有3.8×10⁶个显微注射分子。该技术避免了病毒诱导显微注射中遇到的某些问题。
