Urology Institute, University Hospitals Case Medical Center, Cleveland, OH; Department of Urology, Case Western Reserve University School of Medicine, Cleveland, OH.
Urology Institute, University Hospitals Case Medical Center, Cleveland, OH; Department of Urology, Case Western Reserve University School of Medicine, Cleveland, OH.
Urology. 2014 Aug;84(2):490.e1-6. doi: 10.1016/j.urology.2014.04.024. Epub 2014 Jun 21.
To develop and test the efficacy of an implantable bladder electrode device that can be used with the Neurometer electrodiagnostic stimulator to assess fiber-specific afferent bladder sensation in the mouse.
We constructed a ball-tipped platinum electrode and surgically implanted it into the mouse bladder. The Neurometer was connected to the electrode to apply selective nerve fiber stimuli (250 Hz for Aδ fibers and 5 Hz for C fibers) of increasing intensities to the bladder mucosa in the mouse to determine bladder sensory threshold (BST) values. Using 58 female C57BL/6J mice, we measured the temporal and interobserver consistency of BST measurements, the effects of intravesical administration of lidocaine and resiniferatoxin on the BST, and the effects of our device on voiding behavior and bladder mucosal integrity.
BST values at 250 and 5 Hz did not vary significantly when measured 2, 4, and 6 days after device implantation, or when obtained by 2 blinded independent observers. Intravesical lidocaine yielded a transient increase in BST values at both 250 Hz and 5 Hz, whereas resiniferatoxin yielded a significant increase only at the 5 Hz stimulus frequency after 24 hours. Moderately increased micturition frequency and decreased volume per void were observed 4 and 6 days after device implantation. Histology revealed mild inflammatory changes in the area of the bladder adjacent to the implanted BST device.
Assessment of neuroselective bladder sensation in mice is feasible with our device, which provides reproducible BST values for autonomic bladder afferent nerve fibers.
开发和测试一种可与 Neurometer 电诊断刺激器一起使用的植入式膀胱电极设备，以评估小鼠纤维特异性传入膀胱感觉。
我们构建了一个球端铂电极，并将其手术植入小鼠膀胱。将 Neurometer 连接到电极上，以向小鼠膀胱黏膜施加逐渐增强的选择性神经纤维刺激（250 Hz 用于 Aδ 纤维，5 Hz 用于 C 纤维），以确定膀胱感觉阈值（BST）值。使用 58 只雌性 C57BL/6J 小鼠，我们测量了 BST 测量的时间和观察者间一致性、膀胱内给予利多卡因和瑞芬太尼对 BST 的影响，以及我们的设备对排尿行为和膀胱黏膜完整性的影响。
在植入设备后 2、4 和 6 天测量时，或由 2 位盲法独立观察者测量时，250 Hz 和 5 Hz 的 BST 值没有显著差异。膀胱内利多卡因在 250 Hz 和 5 Hz 刺激频率下均导致 BST 值短暂升高，而瑞芬太尼仅在 24 小时后在 5 Hz 刺激频率下导致 BST 值显著升高。植入设备后 4 天和 6 天观察到排尿频率中度增加和每次排尿量减少。组织学显示植入 BST 设备附近的膀胱区域有轻度炎症变化。
我们的设备可用于评估小鼠的神经选择性膀胱感觉，可提供自主膀胱传入神经纤维的可重复 BST 值。
