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OCT3/4的表达与胃癌的侵袭相关。

OCT3/4 expression is correlated with the invasion of gastric carcinoma.

作者信息

Li Na, Wang Weiqiang, Xu Bin, Gong Hongyun

机构信息

Department of Oncology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

Department of Gastroenterology, 281st Hospital of the PLA, Qinhuangdao, Hebei 066100, P.R. China.

出版信息

Oncol Lett. 2014 Jul;8(1):12-16. doi: 10.3892/ol.2014.2112. Epub 2014 May 7.

DOI:10.3892/ol.2014.2112
PMID:24959212
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4063603/
Abstract

The present study aimed to evaluate the effect of OCT3/4 on the invasion and metastasis ability of gastric cancer. First, the expression level of OCT3/4 was detected in gastric cancer tissues of different tumor-node-metastasis stages. Furthermore, the correlation between the expression of OCT3/4 and the invasion ability of gastric cancer cells, and the probable regulatory mechanism were observed by RNA interference of OCT3/4 in gastric cancer cell strain MKN28, so as to provide the molecular mechanism for the occurrence and development of gastric cancer. The present study found the expression of OCT3/4 in gastric carcinoma tissues (22.56±8.72%) was markedly higher compared with that in para-cancer tissue (1.12±0.18%) (P<0.01). The expression of OCT3/4 was associated with the infiltration degree, and demonstrated an increasing tendency from Tis-T4 stages or from N0-N3. The expression of OCT3/4 in M0 tissues was markedly lower than that in M1 tissues (P<0.01). The level of OCT3/4 was markedly decreased following transfection with OCT3/4 small interfering (si)RNA (P<0.01). The number of cell clones was reduced in a dose-dependent manner following transfection with increasing levels of siRNA, and the number of cells that permeated through the filter membrane was also decreased. It may be concluded that the expression of OCT3/4 increases along with the degree of the infiltration and metastasis of gastric carcinoma, and that OCT3/4 siRNA inhibits the invasion of gastric carcinoma cells.

摘要

本研究旨在评估OCT3/4对胃癌侵袭和转移能力的影响。首先，检测不同肿瘤-淋巴结-转移分期的胃癌组织中OCT3/4的表达水平。此外，通过对胃癌细胞株MKN28进行OCT3/4的RNA干扰，观察OCT3/4表达与胃癌细胞侵袭能力之间的相关性以及可能的调控机制，从而为胃癌的发生发展提供分子机制。本研究发现，胃癌组织中OCT3/4的表达（22.56±8.72%）明显高于癌旁组织（1.12±0.18%）（P<0.01）。OCT3/4的表达与浸润程度相关，从Tis-T4期或从N0-N3期呈上升趋势。M0组织中OCT3/4的表达明显低于M1组织（P<0.01）。用OCT3/4小干扰（si）RNA转染后，OCT3/4水平明显降低（P<0.01）。随着转染的siRNA水平增加，细胞克隆数呈剂量依赖性减少，穿过滤膜的细胞数也减少。可以得出结论，OCT3/4的表达随着胃癌浸润和转移程度的增加而升高，且OCT3/4 siRNA抑制胃癌细胞的侵袭。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2325/4063603/1425dea38c76/OL-08-01-0012-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2325/4063603/50530a331864/OL-08-01-0012-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2325/4063603/8b6f590627cf/OL-08-01-0012-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2325/4063603/87ed849c1aae/OL-08-01-0012-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2325/4063603/1425dea38c76/OL-08-01-0012-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2325/4063603/50530a331864/OL-08-01-0012-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2325/4063603/8b6f590627cf/OL-08-01-0012-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2325/4063603/87ed849c1aae/OL-08-01-0012-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2325/4063603/1425dea38c76/OL-08-01-0012-g03.jpg

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OCT4B1, a novel spliced variant of OCT4, is highly expressed in gastric cancer and acts as an antiapoptotic factor.OCT4B1，一种新型的 OCT4 剪接变体，在胃癌中高表达，并发挥抗凋亡因子的作用。
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