长链非编码 RNA Lnc01614 通过激活 EMT 通路促进胃癌的发生发展。

Long noncoding RNA Lnc01614 promotes the occurrence and development of gastric cancer by activating EMT pathway.

机构信息

Health Management Center, Linyi Central Hospital, Linyi, China.

出版信息

Eur Rev Med Pharmacol Sci. 2018 Mar;22(5):1307-1314. doi: 10.26355/eurrev_201803_14472.

DOI:10.26355/eurrev_201803_14472

PMID:29565488

Abstract

OBJECTIVE

To investigate the expression characteristics of long noncoding ribonucleic acid (lncRNA) lnc01614 in gastric cancer, so as to further investigate its roles in the occurrence and development of gastric cancer and its potential regulatory mechanism.

PATIENTS AND METHODS

Quantitative Real-time polymerase chain reaction (qRT-PCR) was used to detect the expression levels of lnc01614 in 79 pairs of gastric cancer tissues and normal adjacent tissues. The correlations of lnc01614 expression with pathological parameters of gastric cancer and prognoses of patients were also analyzed. QRT-PCR was employed to further verify the expression of lnc01614 in gastric cancer cells. Lnc01614 knockdown expression models were established using small interfering RNA (siRNA) in gastric cancer cell strains (SGC-7901 and AGS). The effect of lnc01614 on the biological function of gastric cancer cells was analyzed by Cell Counting Kit-8 (CCK-8) and transwell invasion and migration assay. Lastly, Western blotting was performed to explore its potential mechanism.

RESULTS

The results of qRT-PCR showed that the expression of lnc01614 in gastric cancer tissues was significantly higher than that in normal tissues. Compared with patients with low expression of lnc01614, patients with high expression of lnc01614 had higher tumor staging, greater lymph node metastasis and distant metastasis rates, and lower overall survival rate. In comparison with the negative control si-NC group, cell proliferation, invasion and migration abilities in lnc01614 knockdown expression group (si-lnc01614) were significantly decreased. Western blotting results indicated that si-lnc01614 group exhibited increased E-cadherin expression, and significantly reduced vimentin and snail expression.

CONCLUSIONS

Lnc01614 was upregulated in gastric cancer and significantly correlated with gastric cancer staging, lymph node metastasis, distant metastasis and poor prognosis. Lnc01614 may promote the proliferation, invasion and migration capabilities of gastric cancer through the regulation of epithelial-mesenchymal transition (EMT) pathway.

摘要

目的

研究长链非编码 RNA（lncRNA）lnc01614 在胃癌中的表达特征，进而探讨其在胃癌发生发展中的作用及其潜在的调控机制。

患者与方法

采用实时荧光定量聚合酶链反应（qRT-PCR）检测 79 对胃癌组织及癌旁正常组织中 lnc01614 的表达水平，分析 lnc01614 表达与胃癌患者病理参数及预后的相关性。采用 qRT-PCR 进一步验证 lnc01614 在胃癌细胞中的表达。采用小干扰 RNA（siRNA）构建胃癌细胞株（SGC-7901 和 AGS）lnc01614 低表达模型，采用细胞计数试剂盒（CCK-8）和 Transwell 侵袭和迁移实验分析 lnc01614 对胃癌细胞生物学功能的影响，采用 Western blot 实验探讨其潜在作用机制。

结果

qRT-PCR 结果显示，lnc01614 在胃癌组织中的表达明显高于癌旁正常组织。与 lnc01614 低表达患者相比，lnc01614 高表达患者的肿瘤分期更高，淋巴结转移和远处转移率更高，总生存率更低。与阴性对照 si-NC 组相比，lnc01614 敲低表达组（si-lnc01614）细胞增殖、侵袭和迁移能力明显降低。Western blot 结果显示，si-lnc01614 组 E-钙黏蛋白表达增加，波形蛋白和 snail 表达明显降低。