Wang An-Cong, Wu Feng-Xia, Gao Yong-Sheng, Sheng Xiu-Gui
Department of Reproductive Medicine, Linyi People's Hospital, Linyi, Shandong 276003, P.R. China ; Department of Gynecologic Oncology, Shandong Cancer Hospital and Institute, Jinan, Shandong 250117, P.R. China.
Department of Anatomy, Shandong University, Jinan, Shandong 250012, P.R. China.
Oncol Lett. 2014 Jul;8(1):438-440. doi: 10.3892/ol.2014.2113. Epub 2014 May 7.
Toll-like receptor (TLR4) 4 is present in numerous cell types and serves as the first point of defense in the innate immune system. Single-nucleotide polymorphisms (SNPs) are present in a number TLR genes and have been associated with various infection and inflammation disorders. Asp299Gly and Thr399Ile, TLR4 SNPs, are associated with tumor progression. In the present study, cases of ovarian cancer were analyzed with regards to Asp299Gly and Thr399Ile of the TLR4 gene. Genotype analysis was performed using DNA from tissue samples from stage I-IV patients with ovarian cancer. DNA from tissue samples was extracted and analyzed by a pyrosequencing method following multiplex polymerase chain reaction. The genotypes of these SNPs were analyzed in the present study in a population of 105 patients, with different types of ovarian cancer, between 2004 and 2012. The allele frequencies for TLR4 Asp299Gly identified in this population were 1.00 (A) and 0.00 (G); for TLR4 Thr399Ile the allele frequencies were; 1.00 (C) and 0.00 (T). For TLR4 Asp299Gly the observed genotype frequency was 1.00 (AA), 0.00 (AG) and 0.0 (GG). In TLR4 Thr399Ile the observed genotype frequencies were 1.00 (CC), 0.00 (CT) and 0.00 (TT). TLR4 Asp299Gly and Thr399Ile alleles were not detected in the patients. These results indicated that the TLR4 299Gly and 399Ile alleles were exhibited at a lower frequency in the ovarian cancer patients that were examined.
Toll样受体4（TLR4）存在于多种细胞类型中，是先天免疫系统的第一道防线。多个TLR基因中存在单核苷酸多态性（SNP），并与各种感染和炎症性疾病相关。TLR4的单核苷酸多态性Asp299Gly和Thr399Ile与肿瘤进展相关。在本研究中，对卵巢癌病例进行了TLR4基因Asp299Gly和Thr399Ile方面的分析。使用来自I-IV期卵巢癌患者组织样本的DNA进行基因分型分析。组织样本的DNA经多重聚合酶链反应后通过焦磷酸测序法提取和分析。在本研究中，于2004年至2012年间对105例患有不同类型卵巢癌的患者群体进行了这些SNP的基因型分析。在该群体中鉴定出的TLR4 Asp299Gly的等位基因频率为1.00（A）和0.00（G）；对于TLR4 Thr399Ile，等位基因频率为1.00（C）和0.00（T）。对于TLR4 Asp299Gly，观察到的基因型频率为1.00（AA）、0.00（AG）和0.0（GG）。在TLR4 Thr399Ile中，观察到的基因型频率为1.00（CC）、0.00（CT）和0.00（TT）。在患者中未检测到TLR4 Asp299Gly和Thr399Ile等位基因。这些结果表明，在所检查的卵巢癌患者中，TLR4 299Gly和399Ile等位基因的出现频率较低。
