Vilaplana Cristina, Prats Clara, Marzo Elena, Barril Carles, Vegué Marina, Diaz Jorge, Valls Joaquim, López Daniel, Cardona Pere-Joan
Unitat de Tuberculosi Experimental (UTE), Fundació Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol, Universitat Autònoma de Barcelona, CIBERES, Badalona, Catalonia, Spain.
Escola Superior d'Agricultura de Barcelona, Departament de Física i Enginyeria Nuclear, Universitat Politècnica de Catalunya, C/Esteve Terradas, Castelldefels, Catalonia, Spain.
PLoS One. 2014 Jun 24;9(6):e100830. doi: 10.1371/journal.pone.0100830. eCollection 2014.
The temporo-spatial relationship between the three organs (lung, spleen and lymph node) involved during the initial stages of Mycobacterium tuberculosis infection has been poorly studied. As such, we performed an experimental study to evaluate the bacillary load in each organ after aerosol or intravenous infection and developed a mathematical approach using the data obtained in order to extract conclusions. The results showed that higher bacillary doses result in an earlier IFN-γ response, that a certain bacillary load (BL) needs to be reached to trigger the IFN-γ response, and that control of the BL is not immediate after onset of the IFN-γ response, which might be a consequence of the spatial dimension. This study may have an important impact when it comes to designing new vaccine candidates as it suggests that triggering an earlier IFN-γ response might not guarantee good infection control, and therefore that additional properties should be considered for these candidates.
在结核分枝杆菌感染初始阶段所涉及的三个器官（肺、脾和淋巴结）之间的时空关系尚未得到充分研究。因此，我们进行了一项实验研究，以评估气溶胶或静脉感染后每个器官中的细菌载量，并利用所获得的数据开发了一种数学方法来得出结论。结果表明，更高的细菌剂量会导致更早的IFN-γ反应，需要达到一定的细菌载量（BL）才能触发IFN-γ反应，并且在IFN-γ反应开始后对细菌载量的控制并非即时生效，这可能是空间维度的结果。这项研究在设计新的候选疫苗方面可能具有重要影响，因为它表明触发更早的IFN-γ反应可能无法保证良好的感染控制，因此这些候选疫苗应考虑其他特性。
