Petrovic L M, Burroughs A, Scheuer P J
Department of Histopathology, Royal Free Hospital, London, UK.
Histopathology. 1989 Mar;14(3):233-43. doi: 10.1111/j.1365-2559.1989.tb02142.x.
The sinusoidal endothelial cells of human liver can be identified by light and electron microscopy, but there appear to be no specific immunocytochemical markers of these cells. Among specific markers available for vascular endothelial cells in general, Ulex europaeus I lectin (UEA I) is the most sensitive. In the present study, 37 liver biopsies were examined for UEA I binding and for Factor VIII related antigen (F VIII RAg) to determine if sinusoidal endothelial cells were positive. The material included normal liver, biopsies from patients with cirrhosis and biopsies in a variety of other liver diseases. Three embryonal human livers were also included in the immunocytochemical analysis. Eleven oesophageal rings obtained at mechanical transection for variceal bleeding in cirrhotic patients were used as control tissue. Sinusoidal endothelial cells of normal liver did not stain with UEA I, but six of seven with alcoholic cirrhosis and only one of 25 non-cirrhotic liver specimens (a case of acute hepatitis with bridging necrosis) were positive. In two of the six cirrhoses the sinusoidal endothelial cells were stained for F VIII RAg as well. Embryonal sinusoidal endothelial cells were stained with UEA I but were negative for F VIII RAg. The results of the study confirm that sinusoidal endothelial cells of normal adult human liver are phenotypically different from those lining blood vessels in other sites. In cirrhosis, positive staining may be related to the transformation of hepatic sinusoids into true capillaries and thus be a marker of the severity of physiological disturbance in the liver.
人类肝脏的窦状隙内皮细胞可通过光学显微镜和电子显微镜识别，但这些细胞似乎没有特异性免疫细胞化学标记物。在一般可用于血管内皮细胞的特异性标记物中，欧洲荆豆凝集素I（UEA I）最为敏感。在本研究中，对37例肝活检组织进行了UEA I结合及因子VIII相关抗原（F VIII RAg）检测，以确定窦状隙内皮细胞是否呈阳性。材料包括正常肝脏、肝硬化患者的活检组织以及各种其他肝脏疾病的活检组织。免疫细胞化学分析还包括三个胚胎期人类肝脏。将11个在肝硬化患者静脉曲张破裂出血时机械切断获取的食管环用作对照组织。正常肝脏的窦状隙内皮细胞不被UEA I染色，但在7例酒精性肝硬化患者中有6例呈阳性，而在25例非肝硬化肝脏标本中只有1例（1例伴有桥接坏死的急性肝炎）呈阳性。在6例肝硬化患者中的2例，其窦状隙内皮细胞也被F VIII RAg染色。胚胎期窦状隙内皮细胞被UEA I染色，但F VIII RAg呈阴性。该研究结果证实，正常成体人类肝脏的窦状隙内皮细胞在表型上与其他部位血管的内皮细胞不同。在肝硬化中，阳性染色可能与肝血窦转变为真正的毛细血管有关，因此可作为肝脏生理紊乱严重程度的一个标记物。
