Raeiszadeh Jahromi Sareh, Mahesh P A, Jayaraj B S, Madhunapantula Subba Rao V, Holla Amrutha D, Vishweswaraiah Sangeetha, Ramachandra Nallur B
Genetics and Genomics Lab, Department of Studies in Zoology, University of Mysore , Manasagangotri, Mysore, Karnataka , India .
J Asthma. 2014 Dec;51(10):1004-13. doi: 10.3109/02770903.2014.938353. Epub 2014 Jul 18.
The development of inflammation in asthma involves an intricate network of cytokines that recruit and activate numerous immune cells. This study was aimed to compare serum levels of IL-10, IL-17F, and IL-33 in asthmatic patients and non-asthmatic controls and correlate cytokine levels to asthma severity and various clinical, spirometric, and laboratory variables.
Using ELISA, serum levels of IL-10, IL-17F, and IL-33 were evaluated in 44 asthmatics (14 mild persistent, 15 moderate persistent, and 15 severe persistent) and 44 controls.
This is one of the first reports showing a significant difference in serum levels of asthma-associated cytokines, anti-inflammatory IL-10, and pro-inflammatory IL-17F and IL-33, in the same subset of asthmatic patients. Our results showed diminished level of IL-10 and elevated levels of IL-17F and IL-33 in asthmatics than in controls (p < 0.001). Assessment of cytokine levels between subjects of different gender, age group, and BMI showed non-significant differences. Correlation analysis of cytokine levels to clinical variables showed that IL-17F is associated negatively to FVC % predicted (forced vital capacity) and FEV1% predicted (forced expiratory volume in one second) and positively to number of allergens sensitized and FEV1 reversibility. A strong negative correlation was found between IL-10 and IL-33 levels (p = 0.001).
Negative correlation between IL-10 and IL-33 levels may reflect a converse relationship between anti-inflammatory and pro-inflammatory cytokines in an individually balanced pattern. The association between IL-17F level and asthmatic phenotypes such as reduced FVC and FEV1, higher degree of sensitization, and post-bronchodilator reversibility needs further assessments.
哮喘炎症的发展涉及一个复杂的细胞因子网络，该网络可募集并激活众多免疫细胞。本研究旨在比较哮喘患者和非哮喘对照者血清中白细胞介素-10（IL-10）、白细胞介素-17F（IL-17F）和白细胞介素-33（IL-33）的水平，并将细胞因子水平与哮喘严重程度以及各种临床、肺功能和实验室指标相关联。
采用酶联免疫吸附测定法（ELISA），对44例哮喘患者（14例轻度持续性、15例中度持续性和15例重度持续性）和44例对照者的血清IL-10、IL-17F和IL-33水平进行评估。
这是首批报告之一，显示在同一组哮喘患者中，与哮喘相关的细胞因子、抗炎性IL-10以及促炎性IL-17F和IL-33的血清水平存在显著差异。我们的结果显示，哮喘患者的IL-10水平降低，IL-17F和IL-33水平升高，与对照组相比差异有统计学意义（p < 0.001）。对不同性别、年龄组和体重指数（BMI）受试者的细胞因子水平进行评估，结果显示无显著差异。细胞因子水平与临床指标的相关性分析表明，IL-17F与预测的用力肺活量（FVC）百分比和预测的一秒用力呼气量（FEV1）百分比呈负相关，与致敏变应原数量和FEV1可逆性呈正相关。IL-10和IL-33水平之间存在强烈的负相关（p = 0.001）。
IL-10和IL-33水平之间的负相关可能反映了抗炎性和促炎性细胞因子在个体平衡模式下的相反关系。IL-17F水平与哮喘表型（如FVC和FEV1降低、致敏程度较高以及支气管扩张剂后可逆性）之间的关联需要进一步评估。
