Aparicio P, López de Castro J A
Department of Immunology, Fundación Jiménez Díaz (C.S.I.C.), Madrid, Spain.
Hum Immunol. 1989 Apr;24(4):295-9. doi: 10.1016/0198-8859(89)90022-0.
A CD3+ CD4- CD8+ cytolytic T-lymphocyte (CTL) clone, CTL 47, could be induced to proliferate in the presence of exogenous interleukin 2 by either HLA-B27.1+ or HLA-DR2+ cells. B27.1-induced proliferation was strongly and equally inhibited by an anti-B27 and by an anti-CD8 monoclonal antibody (MoAb). DR2-induced proliferation was inhibited by the same anti-CD8 MoAb less efficiently and with a different time course than anti-class II blocking, only being significant when the antibody was added ab initio or very early during the assay. These results indicate that CD8 is essential for class I-induced proliferation but that it also enhances class II-induced stimulation of this CTL clone. It is proposed that the necessary role of CD8 in class I-induced proliferation is related to its interaction with the same class I molecule bound by the T-cell receptor. The accessory role in class II-induced proliferation would be due to an additive effect on the avidity of cell adhesion, resulting from interaction of CD8 with the class I antigens on the stimulator cell, or perhaps to a regulatory role of CD8 as a transducer of early signals for T-cell activation.
一个CD3 + CD4 - CD8 + 细胞毒性T淋巴细胞（CTL）克隆，即CTL 47，在存在外源性白细胞介素2的情况下，可被HLA - B27.1 + 或HLA - DR2 + 细胞诱导增殖。B27.1诱导的增殖被抗B27和抗CD8单克隆抗体（MoAb）强烈且同等程度地抑制。DR2诱导的增殖被相同的抗CD8 MoAb抑制的效率较低，且时间进程与抗II类阻断不同，只有在抗体从一开始就加入或在检测过程中非常早期加入时才显著。这些结果表明，CD8对于I类诱导的增殖至关重要，但它也增强了II类对该CTL克隆的刺激作用。有人提出，CD8在I类诱导的增殖中的必要作用与其与T细胞受体结合的同一I类分子的相互作用有关。在II类诱导的增殖中的辅助作用可能是由于CD8与刺激细胞上的I类抗原相互作用对细胞黏附亲和力的累加效应，或者可能是由于CD8作为T细胞活化早期信号转导器的调节作用。
