Aparicio P, Jaraquemada D, López de Castro J A
J Exp Med. 1987 Feb 1;165(2):428-43. doi: 10.1084/jem.165.2.428.
HLA-B27- responder cells were stimulated in vitro with HLA-B27.1+ lymphoblastoid cell lines, and alloreactive CTL clones were obtained by limiting dilution. Three of these clones specifically lysed B27.1+ targets. In addition, they also lysed homozygous DR2 targets with various degrees of efficiency, depending on the Dw specificity of the target cell. All three clones possessed a homogeneous CD3+,CD8+,CD4- phenotype and were also homogeneous upon subcloning. Cold-target inhibition analyses showed mutual inhibition of B27.1 target lysis by DR2 targets and vice versa. Lysis of B27.1 targets was selectively inhibited by anti-class I mAbs. In contrast, lysis of DR2 targets was inhibited only by anti-class II and anti-DR monomorphic antibodies, but not by anti-class I, anti-DQw1, or anti-DP antibodies. The results indicate that these clones display dual recognition for HLA-B27.1 and for HLA-DR2 and suggest that HLA-B27.1 may share at least one epitope that is closely related to some stimulatory Dw determinants present on the HLA-DR2 antigens. Lysis of both B27+ and DR+ targets was inhibited by an anti-CD3 mAb. In contrast, an anti-CD8 antibody selectively inhibited the B27- but not the DR2-directed killing by these clones. The data support a stabilizing role of CD8 through its binding to the same class I (but not class II) molecule on the target cell bound by the T cell antigen receptor.
用HLA - B27.1 +淋巴母细胞系在体外刺激HLA - B27反应细胞，并通过有限稀释获得同种异体反应性CTL克隆。其中三个克隆特异性裂解B27.1 +靶细胞。此外，它们还能以不同程度的效率裂解纯合DR2靶细胞，这取决于靶细胞的Dw特异性。所有三个克隆均具有均一的CD3 +、CD8 +、CD4 -表型，亚克隆后也保持均一性。冷靶抑制分析显示DR2靶细胞对B27.1靶细胞裂解有相互抑制作用，反之亦然。抗I类单克隆抗体选择性抑制B27.1靶细胞的裂解。相反，DR2靶细胞的裂解仅被抗II类和抗DR单态抗体抑制，而不被抗I类、抗DQw1或抗DP抗体抑制。结果表明，这些克隆对HLA - B27.1和HLA - DR2表现出双重识别，提示HLA - B27.1可能至少共享一个与HLA - DR2抗原上某些刺激Dw决定簇密切相关的表位。抗CD3单克隆抗体抑制B27 +和DR +靶细胞的裂解。相反，抗CD8抗体选择性抑制这些克隆对B27而非DR2的定向杀伤。数据支持CD8通过与T细胞抗原受体结合的靶细胞上相同的I类（而非II类）分子结合发挥稳定作用。
