Chen Xiao, Sonchaeng Pichai, Yuvanatemiya Vasin, Nuangsaeng Bunlung, Ai Weiming
a Department of Marine Biotechnology , School of Life Science, Wenzhou Medical University , Wenzhou , PR China and.
b Faculty of Marine Technology , Burapha University , Chanthaburi , Thailand.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(2):947-8. doi: 10.3109/19401736.2014.926499. Epub 2014 Jun 24.
The complete mitochondrial genome of the whitetip reef shark Triaenodon obesus is determined in this study. It is 16,700 bp in length, with the typical gene composition, arrangement and transcriptional orientation in vertebrates. The overall base composition is 31.4% A, 25.8% C, 13.2% G and 29.7% T. Two start codons and two stop codons are found in the protein-coding genes. The 22 tRNA genes ranged from 67 to 75 nucleotides. The tRNA-Ser2 lost the DHU arm and could not be folded to the typical cloverleaf secondary structure. The origin of L-strand replication (OL) sequence was identified between tRNA-Asn and tRNA-Cys genes. The high A+T content of control region is due to a lot of poly A and poly T.
本研究测定了白鳍礁鲨（Triaenodon obesus）的完整线粒体基因组。其长度为16,700 bp，具有脊椎动物典型的基因组成、排列和转录方向。总体碱基组成为31.4%的A、25.8%的C、13.2%的G和29.7%的T。在蛋白质编码基因中发现了两个起始密码子和两个终止密码子。22个tRNA基因的长度在67至75个核苷酸之间。tRNA-Ser2缺失了DHU臂，无法折叠成典型的三叶草二级结构。轻链复制起始点（OL）序列位于tRNA-Asn和tRNA-Cys基因之间。控制区的高A+T含量是由于大量的聚A和聚T。
