用Wnt1a条件培养基处理间充质干细胞可激活毛乳头细胞并促进毛囊再生。
Treatment of MSCs with Wnt1a-conditioned medium activates DP cells and promotes hair follicle regrowth.
作者信息
Dong Liang, Hao Haojie, Xia Lei, Liu Jiejie, Ti Dongdong, Tong Chuan, Hou Qian, Han Qingwang, Zhao Yali, Liu Huiling, Fu Xiaobing, Han Weidong
机构信息
1] Institute of Basic Medicine Science, College of Life Science, Chinese PLA General Hospital, Beijing 100853, China [2].
1] Department of Medical Administration,Chinese PLA General Hospital, Beijing 100853, China [2].
出版信息
Sci Rep. 2014 Jun 25;4:5432. doi: 10.1038/srep05432.
Hair loss (alopecia) is a common problem for people. The dermal papilla is the key signaling center that regulates hair growth and it engage in crosstalk with the microenvironment, including Wnt signaling and stem cells. In this study, we explored the effects of bone marrow mesenchymal stem cell overexpression of Wnt1a on mouse hair follicle regeneration. Wnt-CM accelerated hair follicle progression from telogen to anagen and enhanced the ALP expression in the DP area. Moreover, the hair induction-related genes were upregulated, as demonstrated by qRT-PCR. Wnt-CM treatment restored and increased DP cell expression of genes downregulated by dihydrotestosterone treatment, as demonstrated by qRT-PCR assays. Our study reveals that BM-MSC-generated Wnt1a promotes the DP's ability to induce hair cycling and regeneration.
脱发是人们常见的问题。真皮乳头是调节头发生长的关键信号中心，它与包括Wnt信号和干细胞在内的微环境进行相互作用。在本研究中，我们探究了骨髓间充质干细胞过表达Wnt1a对小鼠毛囊再生的影响。Wnt条件培养基加速了毛囊从休止期到生长期的进程，并增强了真皮乳头区域的碱性磷酸酶表达。此外，qRT-PCR结果表明，与毛发诱导相关的基因上调。qRT-PCR分析表明，Wnt条件培养基处理恢复并增加了经二氢睾酮处理后下调的基因在真皮乳头细胞中的表达。我们的研究表明，骨髓间充质干细胞产生的Wnt1a促进了真皮乳头诱导毛发周期和再生的能力。
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