Brüggemann M, Teale C, Clark M, Bindon C, Waldmann H
Department of Pathology, University of Cambridge, England.
J Immunol. 1989 May 1;142(9):3145-50.
Rat C regions mu, gamma 1, gamma 2a, gamma 2b, gamma 2c, epsilon, and alpha have been characterized by means of chimeric antibody technology. A set of rat/mouse Ag-specific (anti-4-hydroxy-3-nitrophenacetyl) antibodies was constructed that differ only in the H chain constant region but carry identical V region and L chain, both of which are of mouse origin. All rat constant regions could be expressed and m.w. were as expected from the protein sequence. A slight variation in mobility within the IgG subclasses allowed us to establish a hierarchy for the sizes of the four gamma H chains; gamma 2b greater than gamma 1 greater than gamma 2c greater than gamma 2a. Rat IgG2c and IgG2b could be purified on both protein A and protein G while rat IgG2a could only be purified on protein G. Rat IgM and IgG2b were the most potent in C-mediated hemolysis. This was not simply a consequence of the amount of C1q bound because IgG2c bound C1q efficiently but was relatively poor in cell lysis. In ADCC using human effector and target cells, IgG2b and IgG1 were the most effective.
已通过嵌合抗体技术对大鼠的C区μ、γ1、γ2a、γ2b、γ2c、ε和α进行了表征。构建了一组大鼠/小鼠抗原特异性（抗4-羟基-3-硝基苯乙酰）抗体，这些抗体仅在重链恒定区不同，但具有相同的可变区和轻链，两者均源自小鼠。所有大鼠恒定区均可表达，其分子量与蛋白质序列预期一致。IgG亚类内迁移率的轻微差异使我们能够确定四种γ重链大小的等级；γ2b大于γ1大于γ2c大于γ2a。大鼠IgG2c和IgG2b可在蛋白A和蛋白G上纯化，而大鼠IgG2a只能在蛋白G上纯化。大鼠IgM和IgG2b在补体介导的溶血中最有效。这不仅仅是结合C1q量的结果，因为IgG2c能有效结合C1q，但在细胞裂解方面相对较差。在使用人效应细胞和靶细胞的ADCC中，IgG2b和IgG1最有效。
