突变小鼠眼中DNA泄漏的影响。
Implications of DNA leakage in eyes of mutant mice.
作者信息
Ogilvy Alexander J, Shen Defen, Wang Yujuan, Chan Chi-Chao, Abu-Asab Mones S
机构信息
Laboratory of Immunology, National Eye Institute , Bethesda, MA , USA and.
出版信息
Ultrastruct Pathol. 2014 Oct;38(5):335-43. doi: 10.3109/01913123.2014.927406. Epub 2014 Jun 25.
Abstract
Extranuclear DNA (enDNA) is not well studied ultrastructurally in the retinal pigment epithelium (RPE). We analyzed the retina and vastus medialis muscle of four mouse strains that are related to focal retinal degeneration by transmission electron microscopy (TEM) and EM immunolabeling. Evaluation of enDNA would imply the involvements of enDNA is either limited to the affected tissue or generalized in the whole body. Ultrastructural analysis and EM immunolabeling revealed that enDNA was present in the RPE cells but not in the muscle. These data suggest that enDNA could be unique to unhealthy RPE and a potential biomarker for cellular abnormality.
摘要
核外DNA（enDNA）在视网膜色素上皮（RPE）中的超微结构尚未得到充分研究。我们通过透射电子显微镜（TEM）和免疫电镜标记分析了四种与局灶性视网膜变性相关的小鼠品系的视网膜和股内侧肌。对enDNA的评估将意味着enDNA的参与要么局限于受影响的组织，要么在全身普遍存在。超微结构分析和免疫电镜标记显示，enDNA存在于RPE细胞中，但不存在于肌肉中。这些数据表明，enDNA可能是不健康RPE所特有的，并且是细胞异常的潜在生物标志物。
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