Institute of Advanced Biomedical Engineering and Science, TWIns, Tokyo Women's Medical University, Tokyo, Japan.
Biotechnol J. 2014 Jul;9(7):904-14. doi: 10.1002/biot.201300432. Epub 2014 Jun 25.
Substantial progress made in the areas of stem cell research and regenerative medicine has provided a number of innovative methods to repair or regenerate defective tissues and organs. Although previous studies regarding regenerative medicine, especially those involving induced pluripotent stem cells, have been actively promoted in the past decade, there remain some challenges that need to be addressed in order to enable clinical applications. Designed for use in clinical applications, cell sheet engineering has been developed as a unique, scaffold-free method of cell processing utilizing temperature-responsive cell culture vessels. Clinical studies using cell sheets have shown positive outcomes and will be translated into clinical practice in the near future. However, several challenges stand in the way of the industrialization of cell sheet products and the widespread acceptance of regenerative medicine based on cell sheet engineering. This review describes current strategies geared towards the realization of the regenerative medicine approach.
在干细胞研究和再生医学领域取得了重大进展，为修复或再生有缺陷的组织和器官提供了许多创新方法。尽管过去十年中一直在积极推动再生医学方面的研究，特别是涉及诱导多能干细胞的研究，但仍存在一些需要解决的挑战，以便能够进行临床应用。细胞片工程被设计用于临床应用，它是一种独特的、无支架的细胞处理方法，利用温度响应性细胞培养容器。使用细胞片的临床研究显示出了积极的结果，并将在不久的将来转化为临床实践。然而，细胞片产品的产业化和基于细胞片工程的再生医学的广泛接受仍面临一些挑战。本文综述了目前实现再生医学方法的策略。
