Sedighi Gilani Marjan, Boone Matthieu N, Mader Kevin, Schwarze Francis Willis Mathew Robert
Applied Wood Laboratory, Swiss Federal Laboratories for Materials Science and Technology (EMPA), Switzerland.
Department of Physics and Astronomy, Ghent University, Ghent, Belgium.
J Struct Biol. 2014 Aug;187(2):149-157. doi: 10.1016/j.jsb.2014.06.003. Epub 2014 Jun 22.
Incubation of Norway spruce with Physisporinus vitreus and sycamore with Xylaria longipes results in reduction in density of these wood species that are traditionally used for the top and bottom plate of a violin, which follows by enhanced acoustic properties. We used Synchrotron X-ray micro-tomography, to study the three-dimensional structure of wood at the micro-scale level and the alterations of the density distribution after incubation with two white-rot fungi. Micro-tomography data from wood treated at different incubation periods are analyzed and compared with untreated (control) specimens to determine the wood density map and changes at the cell-wall level. Differences between the density of early- and latewood, xylem ray and around bordered pits in both Norway spruce and sycamore are studied. Three-dimensional hyphal networks of the P.vitreus and Xylaria longipes hyphae are visualized inside the cell lumina and their significance on the density of the early- and latewood cells after different incubation periods are discussed. The study illustrates the utility of X-ray micro-tomography for both qualitative and quantitative studies of a wide variety of biological systems and due to its high sensitivity, small structural changes can be quantified.
用玻璃韧革菌处理挪威云杉以及用长柄炭角菌处理悬铃木，会导致这些传统用于制作小提琴面板和背板的木材密度降低，进而增强声学性能。我们使用同步加速器X射线显微断层扫描技术，在微观尺度上研究木材的三维结构以及与两种白腐菌共培养后密度分布的变化。分析不同培养时期处理过的木材的显微断层扫描数据，并与未处理（对照）样本进行比较，以确定木材密度图以及细胞壁水平的变化。研究了挪威云杉和悬铃木早材与晚材、木射线以及具缘纹孔周围密度的差异。在细胞腔内部可视化了玻璃韧革菌和长柄炭角菌的三维菌丝网络，并讨论了它们在不同培养时期对早材和晚材细胞密度的影响。该研究说明了X射线显微断层扫描技术在对各种生物系统进行定性和定量研究方面的实用性，并且由于其高灵敏度，可以对微小的结构变化进行量化。
