Suttitheptumrong Aroonroong, Pattanakitsakul Sa-Nga
Southeast Asian J Trop Med Public Health. 2014 Jan;45(1):47-52.
Densovirus is classified as invertebrate virus belonging to the subfamily Densovirinae of Parvoviridae family. This group of viruses infects only insects and several densoviruses have been isolated from indigenous mosquitoes and mosquito cell lines. A number of mosquitoes, especially Aedes aegypti and Ae. albopictus are important vectors of viruses, which are the major causes of dengue hemorrhagic fever and yellow fever in humans. As densoviruses do not cause any pathology in humans, these viruses have been proposed to be a potential vector for use in biological control of mosquitoes and insects. We report the application of quantitative (q)PCR to determine the amount of densovirus genome in mosquito cell culture supernatant and mosquito. This method is simple, rapid and has a wide dynamic range, and therefore is likely to be useful and applicable in the determination of viral load of other viruses in a variety of biological specimens.
浓核病毒被归类为无脊椎动物病毒，属于细小病毒科浓核病毒亚科。这类病毒仅感染昆虫，并且已经从本地蚊子和蚊子细胞系中分离出了几种浓核病毒。许多蚊子，尤其是埃及伊蚊和白纹伊蚊，是病毒的重要传播媒介，而这些病毒是人类登革出血热和黄热病的主要病因。由于浓核病毒不会在人类中引起任何病变，因此有人提出这些病毒可作为控制蚊子和昆虫的潜在生物防治载体。我们报告了定量（q）PCR在测定蚊子细胞培养上清液和蚊子中浓核病毒基因组数量方面的应用。该方法简单、快速且具有较宽的动态范围，因此可能在测定各种生物样本中其他病毒的病毒载量方面有用且适用。
