Pattanakitsakul Sa-Nga, Boonnak Kobporn, Auethavornanan Kusuma, Jairungsri Aroonroong, Duangjinda Thaneeya, Puttatesk Punjaporn, Thongrungkiat Supatra, Malasit Prida
Division of Medical Molecular Biology, 12th FI Adulyadej Vikrom Building, Faculty of Medicine Siriraj Hospital, Mahidol University, Prannok Road, Bangkok Noi, Bangkok 10700, Thailand.
Southeast Asian J Trop Med Public Health. 2007 Mar;38(2):283-93.
A new densovirus was isolated and characterized in laboratory strains of Toxorhynchites splendens. The virus was detected by polymerase chain reaction (PCR) from mosquitoes reared in our laboratory. PCR fragments from each mosquito were compared by single strand conformation polymorphism (SSCP) assay and found to be indistinguishable. Thus, it is likely the densoviruses from these mosquitoes contain homologous nucleotide sequences. The PCR fragment corresponding to a 451 bp densovirus structural gene segment from each of 5 mosquitoes had 100% identical nucleotide sequences. Phylogenetic analysis of the structural gene sequence suggests the newly isolated densovirus is more closely related to Aedes aegypti densovirus (AaeDNV) than to Aedes albopictus densovirus (AalDNV). Analysis of offspring and predated larvae suggests that vertical and horizontal transmission are responsible for chronic infections in this laboratory strain of Toxorhynchites splendens. The virion DNA is 4.2 kb in size, is closely related to, but distinct from, known densoviruses in the genera Brevidensovirus and Contravirus. Thevirus is tentatively named Toxorhynchites splendens densovirus (TsDNV).
在华丽巨蚊实验室品系中分离并鉴定出一种新的浓核病毒。通过聚合酶链反应（PCR）从我们实验室饲养的蚊子中检测到该病毒。通过单链构象多态性（SSCP）分析比较了每只蚊子的PCR片段，发现它们无法区分。因此，这些蚊子的浓核病毒可能含有同源核苷酸序列。来自5只蚊子的对应于451 bp浓核病毒结构基因片段的PCR片段具有100%相同的核苷酸序列。对结构基因序列的系统发育分析表明，新分离的浓核病毒与埃及伊蚊浓核病毒（AaeDNV）的关系比与白纹伊蚊浓核病毒（AalDNV）的关系更密切。对后代和被捕食幼虫的分析表明，垂直传播和水平传播是导致华丽巨蚊实验室品系慢性感染的原因。病毒粒子DNA大小为4.2 kb，与短浓核病毒属和反病毒属中的已知浓核病毒密切相关，但又有所不同。该病毒暂命名为华丽巨蚊浓核病毒（TsDNV）。
